Last updated: 2024-02-05

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Hello! I am first loading all the beautiful libraries I will need.

#library(Biobase)
library(edgeR)#
library(limma)#
library(RColorBrewer)
# library(mixOmics)
#library(VennDiagram)
#library(HTSFilter)
library(gridExtra)#
library(reshape2)#
library(devtools)
library(AnnotationHub)
library(tidyverse)
library(scales)
library(biomaRt)#
library(Homo.sapiens)
library(cowplot)#
library(ggrepel)#
library(corrplot)
library(Hmisc)

The next step is to load all the data I will be using. Currently, I am not posting the raw data, but I will release in the future.

This is how I retrieved the gene symbols.

 ###now we add genenames to the geneid###
 
geneid <- rownames(mymatrix) ### pulls the names we have in the counts file
genes <- select(Homo.sapiens, keys=geneid, columns=c("SYMBOL"),
  keytype="ENTREZID")
genes <- genes[!duplicated(genes$ENTREZID),]
mymatrix$genes <- genes

Filtering out low count genes

Filtering the genes that are lowly expressed using several methods.

First method, removing only those rows with zero counts across all samples.

# 
# old.par <- par(mar = c(0, 0, 0, 0))
# par(old.par)
# boxplot(data =RNAseqreads, total~Sample, main = "Boxplots of total reads",xaxt = "n", xlab= "")
# x_axis_labels(labels = samplenames, every_nth = 1, adj=1, srt =90, cex =0.4)
# ggplot(RNAseqreads, x = Sample, y = total)+
#   geom_boxplot()

table(rowSums(mymatrix$counts==0)==72)

FALSE  TRUE 
26487  1908 

This filtering would leave 24931 genes and remove 3464, That is too many leftover genes!

So now to try something a little more stringent using the built in function from the edgeR package.

keep <- filterByExpr.DGEList(mymatrix, group = group)
filter_test <- mymatrix[keep, , keep.lib.sizes=FALSE]
dim(filter_test)
[1] 17189    72

This method effectively uses a cutoff off that leaves 14448 genes. The cutoff is determined by keeping genes that have a count-per-million (CPM) above 10, (the default minimum set) in 6 samples. A set is determined using the design matrix.
For my design, I grouped my 72 samples into sets of 6, one set includes each individual + a specific treatment + a specific time.

The beginning cutoff-standard in our lab is to start by using the rowMeans >0 cutoff on the log10 of cpm.

cpm <- cpm(mymatrix)
lcpm <- cpm(mymatrix, log=TRUE)  ### for determining the basic cutoffs
dim(lcpm)
[1] 28395    72
L <- mean(mymatrix$samples$lib.size) * 1e-6
M <- median(mymatrix$samples$lib.size) * 1e-6
c(L, M)
[1] 21.77031 19.77884
filcpm_matrix <- subset(lcpm, (rowMeans(lcpm)>0))


write.csv(filcpm_matrix, "data/filtered_cpm_counts.csv")
dim(filcpm_matrix)
[1] 14084    72
##method 2 with rowMeans
row_means <- rowMeans(lcpm)
x <- mymatrix[row_means > 0,]
dim(x)
[1] 14084    72
write.csv(x$counts, "data/raw_counts.csv")
saveRDS(x,"data/filtermatrix_x.RDS")

Both of the above methods leave 14823 genes from 28,395. I prefer the second method, which keeps the DGEList format of the data.

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now I will produce the RIN x sample plots:###

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Principal Component Analysis

PCA was done using code adopted from J. Blischak.



###  Vehicle 

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###  Daunorubicin 

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###  Doxorubicin 

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###  Epirubicin 

Version Author Date
c2db62c reneeisnowhere 2023-06-16
cdd9374 reneeisnowhere 2023-02-10


###  Mitoxantrone 

Version Author Date
c2db62c reneeisnowhere 2023-06-16
cdd9374 reneeisnowhere 2023-02-10


###  Trastuzumab 

Version Author Date
c2db62c reneeisnowhere 2023-06-16
cdd9374 reneeisnowhere 2023-02-10

PCA of all 72 samples

Warning: `qplot()` was deprecated in ggplot2 3.4.0.
This warning is displayed once every 8 hours.
Call `lifecycle::last_lifecycle_warnings()` to see where this warning was
generated.

Version Author Date
cdd9374 reneeisnowhere 2023-02-10
             samplenames indv         drug time RIN group        PC1
Da.1.3h  MCW_RM_R_11_S72    1 Daunorubicin   3h 9.3     1 -18.801867
Do.1.3h  MCW_RM_R_12_S1_    1  Doxorubicin   3h 9.8     2 -12.477726
Ep.1.3h  MCW_RM_R_13_S2_    1   Epirubicin   3h 9.8     3 -12.502827
Mi.1.3h  MCW_RM_R_14_S3_    1 Mitoxantrone   3h  10     4 -10.669231
Tr.1.3h  MCW_RM_R_15_S4_    1  Trastuzumab   3h 9.6     5 -11.883176
Ve.1.3h  MCW_RM_R_16_S5_    1      Vehicle   3h 9.9     6 -14.354941
Da.1.24h MCW_RM_R_17_S6_    1 Daunorubicin  24h 7.8     7 133.112599
Do.1.24h MCW_RM_R_18_S7_    1  Doxorubicin  24h 8.7     8 127.043322
Ep.1.24h MCW_RM_R_19_S8_    1   Epirubicin  24h 8.6     9 140.502625
Mi.1.24h MCW_RM_R_20_S9_    1 Mitoxantrone  24h 9.1    10  33.538711
Tr.1.24h MCW_RM_R_21_S10    1  Trastuzumab  24h 9.4    11  -8.639401
Ve.1.24h MCW_RM_R_22_S11    1      Vehicle  24h 9.3    12 -10.980966
Da.2.3h  MCW_RM_R_23_S12    2 Daunorubicin   3h 9.4     1 -56.228403
Do.2.3h  MCW_RM_R_24_S13    2  Doxorubicin   3h 9.6     2 -54.025883
Ep.2.3h  MCW_RM_R_25_S14    2   Epirubicin   3h 9.9     3 -52.539005
Mi.2.3h  MCW_RM_R_26_S15    2 Mitoxantrone   3h 9.4     4 -46.610204
Tr.2.3h  MCW_RM_R_27_S16    2  Trastuzumab   3h 9.9     5 -56.868667
Ve.2.3h  MCW_RM_R_28_S17    2      Vehicle   3h 9.8     6 -56.481720
Da.2.24h MCW_RM_R_29_S18    2 Daunorubicin  24h 9.5     7 116.277807
Do.2.24h MCW_RM_R_30_S19    2  Doxorubicin  24h 9.9     8 132.058008
Ep.2.24h MCW_RM_R_31_S20    2   Epirubicin  24h 9.6     9  38.091266
Mi.2.24h MCW_RM_R_32_S21    2 Mitoxantrone  24h 9.9    10 -24.495348
Tr.2.24h MCW_RM_R_33_S22    2  Trastuzumab  24h 8.8    11 -57.574004
Ve.2.24h MCW_RM_R_34_S23    2      Vehicle  24h 9.5    12 -62.681169
Da.3.3h  MCW_RM_R_35_S60    3 Daunorubicin   3h 9.3     1 -39.816800
Do.3.3h  MCW_RM_R_36_S61    3  Doxorubicin   3h  10     2 -37.633581
Ep.3.3h  MCW_RM_R_37_S62    3   Epirubicin   3h 9.3     3 -45.111289
Mi.3.3h  MCW_RM_R_38_S63    3 Mitoxantrone   3h 9.6     4 -48.565611
Tr.3.3h  MCW_RM_R_39_S64    3  Trastuzumab   3h 9.7     5 -45.188098
Ve.3.3h  MCW_RM_R_40_S65    3      Vehicle   3h 9.4     6 -51.171067
Da.3.24h MCW_RM_R_41_S66    3 Daunorubicin  24h 8.9     7 106.948600
Do.3.24h MCW_RM_R_42_S67    3  Doxorubicin  24h 7.5     8 108.983709
Ep.3.24h MCW_RM_R_43_S68    3   Epirubicin  24h 8.6     9 130.765696
Mi.3.24h MCW_RM_R_44_S69    3 Mitoxantrone  24h 8.5    10  -6.504792
Tr.3.24h MCW_RM_R_45_S70    3  Trastuzumab  24h 8.6    11 -52.538480
Ve.3.24h MCW_RM_R_46_S71    3      Vehicle  24h 9.3    12 -60.196789
Da.4.3h  MCW_RM_R_47_S24    4 Daunorubicin   3h 9.6     1 -25.797717
Do.4.3h  MCW_RM_R_48_S25    4  Doxorubicin   3h 9.6     2 -31.029147
Ep.4.3h  MCW_RM_R_49_S26    4   Epirubicin   3h 9.4     3 -27.951634
Mi.4.3h  MCW_RM_R_50_S27    4 Mitoxantrone   3h 9.7     4 -27.542076
Tr.4.3h  MCW_RM_R_51_S28    4  Trastuzumab   3h 9.9     5 -33.226852
Ve.4.3h  MCW_RM_R_52_S29    4      Vehicle   3h  10     6 -28.909159
Da.4.24h MCW_RM_R_53_S30    4 Daunorubicin  24h 9.4     7 116.151811
Do.4.24h MCW_RM_R_54_S31    4  Doxorubicin  24h 9.6     8 119.226876
Ep.4.24h MCW_RM_R_55_S32    4   Epirubicin  24h 9.4     9 111.579718
Mi.4.24h MCW_RM_R_56_S33    4 Mitoxantrone  24h 9.5    10   7.516338
Tr.4.24h MCW_RM_R_57_S34    4  Trastuzumab  24h 9.8    11 -27.393690
Ve.4.24h MCW_RM_R_58_S35    4      Vehicle  24h 9.5    12 -26.107083
Da.5.3h  MCW_RM_R_59_S36    5 Daunorubicin   3h 9.6     1 -24.048107
Do.5.3h  MCW_RM_R_60_S37    5  Doxorubicin   3h 9.7     2 -24.634225
Ep.5.3h  MCW_RM_R_61_S38    5   Epirubicin   3h 9.6     3 -29.927845
Mi.5.3h  MCW_RM_R_62_S39    5 Mitoxantrone   3h 9.9     4 -33.906761
Tr.5.3h  MCW_RM_R_63_S40    5  Trastuzumab   3h 9.6     5 -39.325444
Ve.5.3h  MCW_RM_R_64_S41    5      Vehicle   3h  10     6 -36.648464
Da.5.24h MCW_RM_R_65_S42    5 Daunorubicin  24h 8.3     7  91.009431
Do.5.24h MCW_RM_R_66_S43    5  Doxorubicin  24h 8.4     8  88.675805
Ep.5.24h MCW_RM_R_67_S44    5   Epirubicin  24h 9.4     9  70.928861
Mi.5.24h MCW_RM_R_68_S45    5 Mitoxantrone  24h 9.7    10  10.795178
Tr.5.24h MCW_RM_R_69_S46    5  Trastuzumab  24h  10    11 -39.481396
Ve.5.24h MCW_RM_R_70_S47    5      Vehicle  24h  10    12 -37.407749
Da.6.3h  MCW_RM_R_71_S48    6 Daunorubicin   3h 9.6     1 -38.123104
Do.6.3h  MCW_RM_R_72_S49    6  Doxorubicin   3h 9.6     2 -43.857325
Ep.6.3h  MCW_RM_R_73_S50    6   Epirubicin   3h 9.7     3 -42.874754
Mi.6.3h  MCW_RM_R_74_S51    6 Mitoxantrone   3h 9.5     4 -43.386618
Tr.6.3h  MCW_RM_R_75_S52    6  Trastuzumab   3h 9.8     5 -52.613108
Ve.6.3h  MCW_RM_R_76_S53    6      Vehicle   3h 9.5     6 -49.957975
Da.6.24h MCW_RM_R_77_S54    6 Daunorubicin  24h 9.8     7  88.506102
Do.6.24h MCW_RM_R_78_S55    6  Doxorubicin  24h 8.6     8  32.474305
Ep.6.24h MCW_RM_R_79_S56    6   Epirubicin  24h 8.9     9  28.195264
Mi.6.24h MCW_RM_R_80_S57    6 Mitoxantrone  24h 9.1    10 -16.793355
Tr.6.24h MCW_RM_R_81_S58    6  Trastuzumab  24h 9.3    11 -48.426347
Ve.6.24h MCW_RM_R_82_S59    6      Vehicle  24h 9.6    12 -48.471055
                 PC2         PC3         PC4         PC5         PC6
Da.1.3h  -61.6492431  40.7087307  -5.3120903  24.4018905 -35.4444449
Do.1.3h  -73.1898070  27.3505327   3.8688884 -18.1052473 -19.4252416
Ep.1.3h  -66.3749523  32.9124408   1.2376582  18.0762710 -42.4344457
Mi.1.3h  -72.8150505  23.0530849  10.2568266  -7.4645061 -24.6681542
Tr.1.3h  -79.0376832  10.2595124  17.2423744 -31.9316183 -11.3241836
Ve.1.3h  -75.2963086  17.7279049   2.3341441 -37.4209985 -16.5916909
Da.1.24h -41.0164141  20.2282172  23.5191002 -23.7072969   9.6461308
Do.1.24h -45.1152922  10.9557441   9.0092013 -36.6981861   3.6743944
Ep.1.24h -34.9090068   0.9833687  32.7871174 -18.3866562   6.3054880
Mi.1.24h -82.2447484 -12.9914585  19.1613846 -42.6209357  -0.5124787
Tr.1.24h -82.6021742 -10.6353249  39.5323823 -40.0857874  12.0923404
Ve.1.24h -91.3265517   1.2672540  26.2234796 -43.8615491  18.6989015
Da.2.3h   63.1090955  28.9080209   3.5356043 -13.9094355 -37.6027975
Do.2.3h   67.9737849  17.2141258  -2.6490686 -47.7487786 -32.9519498
Ep.2.3h   59.3094530  21.1733532  11.0106949  -7.3427097 -50.1148519
Mi.2.3h   58.4795045  12.2405645  26.9640588 -21.4948193 -38.7602209
Tr.2.3h   77.4375391   8.0171252  -5.8523566 -44.2040959 -52.5268371
Ve.2.3h   61.3972180  -1.6863081  -4.8373428 -59.2576074 -13.2879881
Da.2.24h  65.8074456  17.9008114  12.0888708 -18.9646667   2.2138918
Do.2.24h  72.0883536  50.9110380  45.1772733 -37.0334774  36.5553703
Ep.2.24h 106.5998472 193.0345778  56.4013289   9.7790075  50.7488558
Mi.2.24h  52.7566106 -31.5414202 -12.7817910 -35.7989480 -29.3531796
Tr.2.24h  64.0812894 -15.2488653   2.3747760 -32.7440413 -23.4879717
Ve.2.24h  78.5170669  -1.3572652 -24.6341113 -37.7581165 -28.0481219
Da.3.3h   19.9614084   5.9239374  14.7281591  47.6112169  28.9324932
Do.3.3h    7.8253191  -9.6686889   6.4248512  -2.7634404  33.5284259
Ep.3.3h   20.3229898   2.9691142  -0.2989686  59.6256134   6.2421183
Mi.3.3h   30.5500277   3.7254904 -16.1973158  50.0678336  12.9540941
Tr.3.3h   13.7059119 -22.5964128  -6.9278698   0.9766313  25.8637186
Ve.3.3h   25.4232524 -24.6889608  -8.3115384  31.5316688  27.6523982
Da.3.24h  28.7329100 -32.9778627 -20.1118961  21.2387101  -0.6978308
Do.3.24h  51.0044839 -18.4055828 -60.2700910  -5.8025793  29.1942407
Ep.3.24h  43.1433685 -27.5808293  -7.4292516  17.9027540  15.1888979
Mi.3.24h  15.2518542 -42.0134510 -29.6856072  -4.1874824  19.1703708
Tr.3.24h  22.9564059 -23.9195218 -27.9962855 -22.7737881  41.6145547
Ve.3.24h  31.6224811 -17.7337474 -17.5317764 -14.3245894  40.2255084
Da.4.3h  -53.1831700  45.7406998 -14.2156228  25.0384043  -7.0049852
Do.4.3h  -43.0362921  30.8573073 -30.8666478  17.6141940  -4.3506028
Ep.4.3h  -47.4041166  37.4960667 -20.6051516  21.4717734 -12.4520530
Mi.4.3h  -41.3609174  22.1618279 -17.6614901  16.7612270  -6.2533150
Tr.4.3h  -46.1382580  11.3959103 -17.5965024   6.1668565   0.1074265
Ve.4.3h  -54.6024392   9.0981483  -1.8320771   0.5903865  -0.8654210
Da.4.24h  -6.5592155  11.9169585 -25.0294562  20.9353047 -22.1662891
Do.4.24h -17.4354106   1.0574558 -17.2682872  12.7199549 -17.3123361
Ep.4.24h  -5.5299043  19.1272634 -44.6945761  28.9163739 -28.6026630
Mi.4.24h -50.4387048 -10.7922412 -26.5694163  -7.6309107  -5.4701739
Tr.4.24h -50.2752526  -8.5039610  13.3415638   1.3534998   9.9142568
Ve.4.24h -60.8006128  -1.8945272  -1.5736303 -12.8309227  29.0580385
Da.5.3h    6.7933390   0.3645790  59.8524822  60.1356890   4.0877223
Do.5.3h   -2.7541279 -20.3012559  47.0771995  24.9288013   2.8323205
Ep.5.3h    9.9981745 -17.4706010  52.8916850  68.3392347 -22.9182749
Mi.5.3h   11.1139154  -6.3407268  39.3129692  49.1209884 -16.2227417
Tr.5.3h   13.8155816 -38.8645667  45.6772161  23.2817072   0.3146050
Ve.5.3h    6.8356950 -42.9513097  77.7804805   6.9769513 -12.7166830
Da.5.24h  29.0409819 -43.2641481  51.8032440   9.9729007 -13.9267843
Do.5.24h  33.2312625 -44.8981540  -6.0575706  23.5915280 -15.6351533
Ep.5.24h   9.0876488 -64.3124744  23.9367591  14.6739001  -7.8888171
Mi.5.24h  -0.4314044 -55.0248994  29.8297633   0.7353230 -15.3129313
Tr.5.24h   9.9668118 -45.1903867  58.1663438  -3.0260323  20.4266376
Ve.5.24h   3.8399573 -40.2520754  58.0660245 -27.7372162  28.3329415
Da.6.3h    2.6713745  31.0169972 -23.2336363  26.7462346  -5.4957742
Do.6.3h   -6.5062072   6.7747938 -21.7962449   5.1281970  18.5941625
Ep.6.3h   -9.1255573  18.6282730 -33.2849032   3.4916878  15.2108681
Mi.6.3h   -5.7918813  18.9820913 -28.7073388  16.2047006   8.0850111
Tr.6.3h   -3.3827229  -2.0195844 -31.6912000  10.3689165  27.8768091
Ve.6.3h  -13.1731992   0.9808383 -25.0286675   5.7323649  24.0399987
Da.6.24h  15.5774947 -17.6292725 -30.3731789  11.1195258  -7.4914720
Do.6.24h  18.1399729 -19.0900355 -77.7657390   7.4723735 -17.0806294
Ep.6.24h  11.5482092  -8.6985621 -69.5770292   9.8454710  -9.1505919
Mi.6.24h   2.5146436 -20.0503952 -55.3810406 -12.3708239  13.3173502
Tr.6.24h   2.7562782  -9.8261236 -24.0267721 -34.9929863  59.9114486
Ve.6.24h  -1.4823356  -2.6431594 -25.9503651  -5.6658178  22.9382905
                 PC7         PC8         PC9        PC10        PC11
Da.1.3h    0.1170394 -21.5353752  21.6919896  -9.6709753  21.5344659
Do.1.3h   -3.5441132 -11.3852137   8.2628842 -12.0376119   3.3545955
Ep.1.3h    5.6388639 -31.8311101   4.8278739   7.0515874  28.0686564
Mi.1.3h    2.8080430 -12.7515596   5.2245798  -9.8263165  13.5584165
Tr.1.3h    6.8138544  -7.2326192   4.1070361  -9.0248653  11.4270568
Ve.1.3h    3.7807028   1.3987773  -1.4189018 -14.2131906  -5.1999564
Da.1.24h -20.8369171   6.3162442  51.5703829   4.5982064 -20.7987596
Do.1.24h -15.3444622   0.4502778  45.6241207   2.6079680 -33.9218741
Ep.1.24h -13.6013881 -17.5661872  42.4626064  16.5832746  -6.3210058
Mi.1.24h  30.7767426  -9.0164273  10.1886232 -37.4956946   0.9747284
Tr.1.24h   5.5518555 -13.4696666   7.0983699  -9.7840799  14.0424422
Ve.1.24h  -4.7165160  -7.0015962   6.7896916 -19.0941142   0.5784218
Da.2.3h  -25.8830442 -22.1882433  17.4084838  -0.1517254   1.1027410
Do.2.3h  -24.8615080  11.3844625   1.3878642  -9.8452520 -24.6140049
Ep.2.3h  -30.3761213 -16.8647153  -5.0176761   0.4567520  -5.6018652
Mi.2.3h  -20.6491889   0.3014943   3.1060737 -10.5876876  -6.6374538
Tr.2.3h  -14.0864183   2.7833936  -9.6222374   6.0124637 -10.7044041
Ve.2.3h  -11.9817739   6.4377882  -5.4049382   5.6331056 -13.7885213
Da.2.24h -17.6575868  28.1543116 -10.6453726 -10.2114160  55.5842987
Do.2.24h -45.5341892  40.0335778 -43.9678262 -23.0148194  50.6970956
Ep.2.24h  94.2114564 -26.6242234 -12.4261175   2.2905854 -24.1070264
Mi.2.24h  35.2521902 -12.6861693  -4.4350681 -21.5712309  19.0941449
Tr.2.24h   8.1979259 -16.0503145  13.5261003  20.9710354  15.1029169
Ve.2.24h  12.6381003   2.5000711  20.6270428  14.1573377   4.3161170
Da.3.3h  -34.5444999 -16.5384215  20.5687021 -36.8546299 -17.3299702
Do.3.3h  -36.1029916 -20.6615819 -19.4210472 -26.9165025 -22.9828679
Ep.3.3h  -19.5832512 -36.4882195  19.0694744  -9.0744564   0.3048177
Mi.3.3h  -18.6350463 -24.7268373  21.2960403 -24.1298302  -7.9236249
Tr.3.3h  -19.7571697 -29.0918214 -36.7311933  -7.8835722 -17.0091409
Ve.3.3h   -7.9313613 -18.8120108   6.3646311 -10.5201155  -9.1086368
Da.3.24h  -4.8288374 -26.9041123   2.2983270  -9.9658512  16.0796859
Do.3.24h -19.1328884 -10.3983894   2.3236123 -17.6351489  -3.2058427
Ep.3.24h -29.9269122 -28.6405930 -16.1878829   5.3571665  22.6782995
Mi.3.24h  31.6402572 -29.7957202 -17.1969316 -34.8710162  11.9380476
Tr.3.24h  -6.8540337 -15.0597484  -8.5861332  -7.2545019 -10.6498157
Ve.3.24h -14.2977985 -35.5353222   0.7258584  29.8598768  -2.2384838
Da.4.3h  -17.4585740   1.0090664 -19.3200580   3.2162208  -1.5784595
Do.4.3h  -11.4699855  11.2621027 -21.8766782   5.6247673  -8.3618864
Ep.4.3h  -11.1103534   7.0910910 -16.5360500   6.8284173   0.8305291
Mi.4.3h   -6.5541138  13.1326026 -25.4240012   2.5039160  -2.5463646
Tr.4.3h   -5.7669176   9.8742145 -36.7045010  14.1635724  -6.5136477
Ve.4.3h   -8.6334875  13.5484680 -41.4887827   9.3516750  -6.5958534
Da.4.24h  -8.1497598   3.0134625 -13.0059721  31.5624015 -14.0055485
Do.4.24h -14.3530122  -4.8270299 -24.4225919  27.4747007 -27.1507484
Ep.4.24h   1.8200936  -9.9303025  -8.2339292  43.7066532  -4.6335427
Mi.4.24h  28.7710774 -11.3599319 -34.8719823  -7.7745476  -7.9013568
Tr.4.24h  -3.5938779  -7.5125099 -28.9998165  19.1488547   2.6672936
Ve.4.24h  -8.4208960   5.8121401 -28.5529042   6.8133957  -6.5770969
Da.5.3h   -3.4683796  16.2131144  21.8436721 -10.7794698   8.0513781
Do.5.3h   -2.9814041  -5.4014449 -11.7097658   8.8971159   2.8975872
Ep.5.3h    1.9905498   3.6882549   3.5885525   5.7382159  16.1967051
Mi.5.3h    3.4627076  16.6716270  12.4149943  -8.8799241   1.4665003
Tr.5.3h   20.7521502  24.0736453  10.3831163   9.0115592  -2.4700071
Ve.5.3h   12.9816464  41.3090701 -15.9096519  -0.5923424 -35.4268136
Da.5.24h   8.3252350  44.3907541 -12.2936837 -22.8940789 -46.8637043
Do.5.24h  23.1917830   5.8402807  10.9827866   5.3150865   2.8716810
Ep.5.24h  24.6753959 -11.3799922 -11.9321605  -3.3768807 -11.0662669
Mi.5.24h  50.0744730 -10.5682712 -23.7317959  -3.9869769  25.3598379
Tr.5.24h  13.0080654 -17.5042752   8.2197640  46.6782600  19.6567640
Ve.5.24h   8.0032150   0.9724445   1.4356235  44.5737085   3.7459216
Da.6.3h    1.2462335  29.8301651  17.5741271   0.5367939  16.7199084
Do.6.3h   -0.1278696  32.8616213  22.3055098   8.2433217  14.1982251
Ep.6.3h   -4.1460081  21.9357183  11.8860094  17.1803230  10.9100336
Mi.6.3h   -1.6100333  43.3929000  17.4476559  -7.9590669   8.3105164
Tr.6.3h    3.8117053  36.1279841  13.8065453   9.6655799   9.3857878
Ve.6.3h   -4.6767237  42.0799653   7.9790754  -1.1084916   5.0673132
Da.6.24h  11.2308478  16.5507088   5.9440387  -1.1385956   0.3164799
Do.6.24h  42.4976242  -2.0421154  -1.6736118   3.5038426  -9.0793912
Ep.6.24h  31.9741430  16.8216386  13.4058132 -13.1181855 -10.0317483
Mi.6.24h  39.5264403  21.8503525  11.4329537 -27.5917996   1.9391117
Tr.6.24h   6.1797058  -5.9645868  26.6703687  38.2863700   2.1295711
Ve.6.24h   2.2392897  26.2328680  13.8782873   7.2308531  -0.2124027
                PC12        PC13        PC14        PC15         PC16
Da.1.3h  -12.0359096   9.8876923   7.6518665 -13.2430614   6.69416396
Do.1.3h  -23.8244799   3.7976315  -8.3851977 -12.0107715  11.61011482
Ep.1.3h  -17.9188729  13.5336131  -5.7930514 -16.2614148  -2.21524566
Mi.1.3h  -14.7765375   9.7795742 -11.0850106 -11.4175219   2.67310566
Tr.1.3h  -15.1041879  22.2595172 -12.6465083  -2.1551794  -3.41212766
Ve.1.3h  -18.7191239  16.5561711 -19.0046585  -9.7195961  10.71986577
Da.1.24h  27.0365686 -13.8731868  -5.3559718   3.9235917  -5.42531156
Do.1.24h  12.1139639 -18.9593531  -9.7292431  -0.5409027  11.64351058
Ep.1.24h  20.8705973  -1.9148919 -14.0050132   6.2201227 -17.53522801
Mi.1.24h  21.2620250 -18.4253759   7.4530965  -3.9987426   2.52430718
Tr.1.24h  -2.0204443  17.5473999   4.9803293  10.2400566 -18.18965083
Ve.1.24h  -6.3529153  13.1764879  10.1864384  15.5826527  -3.18587377
Da.2.3h  -11.5334311 -14.9490861  26.4163395  10.0774146   1.86886405
Do.2.3h   -4.4142625 -16.7809816  -6.7833796   3.7650910   6.11822380
Ep.2.3h   -5.7557806 -17.8199562  11.0658545  -3.9780401 -17.24673830
Mi.2.3h    2.0672282  -5.0114372   6.2921514   2.8953213 -10.53277679
Tr.2.3h    2.6537151   7.4313103  -8.6849920  -0.3598908 -19.70020078
Ve.2.3h   -5.9404973  -0.2007403 -18.8294766  10.1078816   7.30117862
Da.2.24h  11.5763943  12.9944225   2.5272159   8.6218795   4.63461401
Do.2.24h   7.0831385 -13.9420677   3.5539703 -31.7491703   9.53917177
Ep.2.24h  -7.8377660   8.4834062  -5.1427754   8.3493295  -7.55558089
Mi.2.24h  24.2280437  -6.5996752   9.5726568  -0.6495081   7.49059548
Tr.2.24h  19.0162396  14.4118328   7.7061648   4.6038013   0.41185000
Ve.2.24h  23.7464711  28.0762103   3.3729797  15.0728663   9.72269708
Da.3.3h   13.4792520  -6.9541494  21.8203497  -7.3136791  -4.41973131
Do.3.3h  -20.5453791 -24.6435358 -18.0295768   4.3336864  -5.76059312
Ep.3.3h   11.5243993   8.6738052  11.3631175 -18.6595222   1.76657047
Mi.3.3h   16.5972229   4.5371821   1.1662840 -12.3460391   7.59461054
Tr.3.3h   -5.5242233  -1.5068247 -32.7610945  -4.0941517 -18.12158267
Ve.3.3h   25.2549640  26.6443050 -12.2961454 -12.4570493  -5.00812582
Da.3.24h -17.3889843  15.8287912  -0.1751952  17.2741185  -6.10777066
Do.3.24h -23.6311927  19.5336433  -6.0078243  16.3359054  38.13474982
Ep.3.24h -10.0208437  22.8994760  -6.6161994  -2.3306804 -15.24737680
Mi.3.24h  19.2518471 -10.2039597  -1.7846500  -1.9246445   0.59543325
Tr.3.24h   3.3687640   4.3051223 -15.2997183  13.8184503  10.45914512
Ve.3.24h  -8.7715454   7.6754613   6.9368238 -17.2126152   8.07419972
Da.4.3h   -5.5642281 -12.0660202  24.0918320  10.6425414  12.64094972
Do.4.3h    1.1680866  -4.2991392   6.2061231   5.7347161  19.33178677
Ep.4.3h   -3.0971024  -4.0700775  14.3882614  10.1978359  14.13421384
Mi.4.3h   10.9343469  -0.5818526  -0.3010829   5.8936173   6.88861644
Tr.4.3h   11.0680515   7.4975186  -2.5603188   1.4826829  -0.61413260
Ve.4.3h    5.9590872   7.1887328  -0.7982364   4.6995249  -9.54864822
Da.4.24h  22.2035919  -9.0422486  -0.9342270  -9.0923250  -0.01243867
Do.4.24h  11.4159156 -10.2205713   0.3678659 -12.0402517  -2.88236535
Ep.4.24h  24.5675600   7.2390676 -11.3755643  -0.6181898  10.62605485
Mi.4.24h  29.0812413 -16.3812141  13.7143678  -0.6933072  10.53721899
Tr.4.24h  17.5147392  12.6845366  19.4708195  11.7261386 -16.59891087
Ve.4.24h  13.9660039   7.8367970  21.8995020  30.8811756  -8.98041683
Da.5.3h    3.2705198 -11.4830458   5.3339302  18.1264350  13.55908587
Do.5.3h  -22.5064622 -24.8001101 -28.7435712  20.5730927   6.61907946
Ep.5.3h    7.2909099  -0.5980779 -11.6325159   6.8327993 -11.54747250
Mi.5.3h    9.4931677 -10.6066524  -8.2694244   6.9881321  12.91661768
Tr.5.3h   22.4202869  14.7560032 -21.0892939   1.4580018  12.40771001
Ve.5.3h    5.6133918  26.0586520 -12.1643908 -32.0491050  13.47949904
Da.5.24h -32.2825917  35.1508693  38.6280321 -11.7903099  -0.70911963
Do.5.24h -24.3587501  10.3250505   3.7183812  27.9732305  11.44571883
Ep.5.24h -32.9100844 -22.0340282  12.5472189  11.1401270 -10.85998538
Mi.5.24h  12.2013331 -33.8334419  -8.5314966 -11.4339849   0.31888986
Tr.5.24h  -8.1049783  -8.3095460   9.1711008  -0.8553153   6.65532329
Ve.5.24h -18.7206319 -17.5379554   9.6627785  -8.1859156  12.29396319
Da.6.3h  -13.3600057  -9.9936692  -2.7197693  -2.5586773  -8.25482760
Do.6.3h  -13.2371245  -7.2519578  -8.2922512   2.0268822  -5.14942509
Ep.6.3h  -25.6989257 -16.1733436  -8.2948595  -4.8753530   2.49393062
Mi.6.3h   -0.5592047  -7.8992610 -10.3036038   4.3308712  -7.79165706
Tr.6.3h    7.4540628   7.3339233 -13.2499037   0.4894876 -11.12035507
Ve.6.3h   -0.8159929   4.8550120  -7.7307718   6.4604407 -19.44605523
Da.6.24h -17.0696802 -10.0288153   9.5719509  -6.2154570 -32.33020453
Do.6.24h -24.3597428  -8.9069930  -8.9714085 -20.7606069  -5.09509712
Ep.6.24h -10.6522855  -2.0387434   4.0266899   0.2099668  -4.46701430
Mi.6.24h  16.6031419  -9.6342128   6.4707955  -3.1429867  -0.99763858
Tr.6.24h -14.5651930  -6.6378929  27.6911903 -23.8900812   5.66337923
Ve.6.24h   6.6230884   7.2548730  15.3518933  -8.4658208 -15.51933011
                PC17        PC18         PC19        PC20          PC21
Da.1.3h   -1.4457388  14.5274119  -8.30956126   2.1659929 -3.871476e+00
Do.1.3h    6.1630656  -0.9786024   2.72981288  -0.5396085  2.580660e+00
Ep.1.3h   -1.4051470   9.7020848   0.13154728   7.9308190 -9.464397e-04
Mi.1.3h    1.4952968  -6.8300186   7.25335659   8.7924890  1.471791e+00
Tr.1.3h    3.2648828  -4.9970175   5.29576603  -7.3443284  1.842296e+00
Ve.1.3h   10.1362151  -6.7253423   6.07296588  -3.8899103  2.554046e+00
Da.1.24h -13.2303009  13.8433121  10.70284466  -8.5670488  9.041299e+00
Do.1.24h  -0.4413679  -1.9238424  -8.79160784  -4.0117196 -1.650210e+01
Ep.1.24h  -9.7073028   2.1828191 -13.13990199   1.2608495 -1.783118e+01
Mi.1.24h  -7.1092736 -13.5359086   2.37341544   3.7962391  6.118096e+00
Tr.1.24h   9.0202412   3.3955355  -3.11841073  -2.3232014  5.304028e+00
Ve.1.24h  15.0956978   0.3074195   1.83560834   8.0471567 -1.798597e+00
Da.2.3h   -4.3005109  13.4115862 -13.60780245   6.4942196  2.574837e+00
Do.2.3h    6.4452220 -15.8069778  -0.54348144  -8.6103096  7.413235e+00
Ep.2.3h    2.7635815  -3.0429670 -11.84211117   2.1768583  3.371037e-01
Mi.2.3h   -8.4032584 -16.7624680   5.40720379   6.7492554  4.833274e+00
Tr.2.3h    7.7658334 -17.3986860   8.16963561   5.8683063  3.444400e+00
Ve.2.3h   -1.8756817  -1.6364694   3.47758593 -10.5005444  3.940730e+00
Da.2.24h  -5.9695671   8.5347893  21.94519319 -13.7137811  1.452502e+01
Do.2.24h  27.9900996  10.0421858  -4.37934103   2.0988741 -1.666742e+01
Ep.2.24h  -3.4789811  -5.2161336  -1.69340271  -1.2648911  6.921258e-01
Mi.2.24h -15.4848610  16.0306613  -6.31239455  -5.8668761 -9.529556e+00
Tr.2.24h  -1.3445665  14.3966737  -2.72154868  -1.4267506 -1.083445e+01
Ve.2.24h   6.2087597   7.4697409   2.10357392   3.8300415 -9.209219e+00
Da.3.3h   11.5015533  -6.9942740   4.33735348 -12.4960362  2.091796e+00
Do.3.3h   -3.0239513  19.5024338   5.22778161  -1.5263586  6.099803e+00
Ep.3.3h    1.8975342   3.8324601   4.94204438 -13.0057393 -4.880663e+00
Mi.3.3h    7.9458440 -16.6776501  18.99805077   1.6303162 -4.418965e+00
Tr.3.3h   -1.7463724  19.0117446   6.03295862   9.1207056 -3.606434e+00
Ve.3.3h    3.8629712  -2.4453184   7.67857209 -15.6381686 -5.132658e+00
Da.3.24h -21.2065654  -6.4605282  -1.10567086 -11.9263902  1.687899e+01
Do.3.24h  -0.7191666 -20.7583197 -17.83025180   6.6818684  8.677014e+00
Ep.3.24h -13.1049378 -24.7547052 -29.96437369  10.5764917 -1.280137e+00
Mi.3.24h -17.6953008   2.6493251   1.25618797   9.1399515  5.893184e+00
Tr.3.24h  -0.9308667  25.9970216  -3.42189909  14.7458669 -5.499005e+00
Ve.3.24h -11.8333670   0.9190118  14.41476335  -0.7675895 -5.557428e+00
Da.4.3h  -13.8605417   6.9180531  -8.62429195  -5.3272467 -3.735885e+00
Do.4.3h   -5.2574647  -0.1438763  -3.29022825 -10.9209860 -3.492784e+00
Ep.4.3h  -12.4920384   4.3430703  -5.32614004 -10.5456270 -2.710507e+00
Mi.4.3h   -6.3802889 -12.0143938   3.93947423  -7.8076542 -4.832566e+00
Tr.4.3h   -2.4165804  -7.0716998  -0.39511606 -12.7632984 -4.957258e+00
Ve.4.3h   -5.2778470  -8.6996586  -1.67526788 -16.0540249 -4.529476e+00
Da.4.24h  -0.1915080   7.3932311  12.08105677   2.4944000  1.737008e+01
Do.4.24h   6.1401274  -1.7289484  -4.32384592  11.1251173 -3.676729e-01
Ep.4.24h   8.5530278   3.3043264  15.05538130  16.0189546  1.036654e+00
Mi.4.24h   4.5124224  -5.6960840  -0.12092046  10.7617021  5.921398e+00
Tr.4.24h   7.6376578   3.8855468  -3.49392667   1.4306458  2.360297e+00
Ve.4.24h  12.5500494   1.2265171  -1.66166047   5.6915294  3.583318e+00
Da.5.3h    3.7570661   7.6277870  -8.99638747   3.3763405  7.551910e+00
Do.5.3h    9.5456587  12.6204773   4.56162699   9.2795926  9.369056e+00
Ep.5.3h   12.2313284   0.5521917  -5.83429995   6.0452030  3.801795e+00
Mi.5.3h   13.5060320 -16.8037473   0.33346878  18.1283101  2.611704e+00
Tr.5.3h   13.2924163   2.4556119 -13.51678970 -10.3699082  8.453857e+00
Ve.5.3h  -17.9155783   8.6225759 -23.41928153  -5.5044288  8.274227e+00
Da.5.24h -17.9708498  12.8795781  20.34668298  21.0778564 -6.100288e+00
Do.5.24h  12.5932479   0.4489559  17.23649913  -9.3633576 -1.468552e+01
Ep.5.24h  11.0626435  -9.4566184   1.01802944 -11.9517075 -3.003258e+01
Mi.5.24h -18.9807500 -15.7445409   9.84645113  -1.2922661 -8.707177e+00
Tr.5.24h   5.7799287  -3.0989749   5.43265364   3.2704281  5.132900e+00
Ve.5.24h  -2.7821055  -8.0206777  -0.87822475 -11.6857399  5.713533e+00
Da.6.3h  -17.0705647   6.7723779  -4.30119827   5.3749129 -1.546160e+00
Do.6.3h  -11.8720163   1.4090294   4.21560285  -3.9377319  1.767073e-01
Ep.6.3h  -15.5602011   1.7212873   6.41994849   5.0010429 -5.334953e+00
Mi.6.3h  -11.3392134 -15.8264275   8.92650828  13.7909869 -1.050280e+01
Tr.6.3h   -4.8091088  -1.6408851  -2.22431542   0.8276443 -7.482424e+00
Ve.6.3h   -6.1307303  -4.2099554  -2.62364579   6.2171292 -7.610385e+00
Da.6.24h   5.5052345   7.2968405  -0.05636913 -14.7615192  2.397664e+01
Do.6.24h  21.3436294  10.6042632 -12.81416413 -11.1836935 -2.395516e+00
Ep.6.24h  24.2153271   9.4023564  -1.32821681  -4.4782772  1.667448e+00
Mi.6.24h   5.5233706   4.0559644 -10.32524367   6.9538701  1.070625e+01
Tr.6.24h   7.4397296 -10.0251528   0.27414247   9.1686679  1.331712e+01
Ve.6.24h   8.0187772  -6.1693885  -8.06245469   4.2260837 -1.694446e+00
                 PC22         PC23         PC24        PC25        PC26
Da.1.3h    0.83844774  -9.71962739  14.33418601  -1.0406108   9.2574629
Do.1.3h   -2.04551898   0.77991399   5.12919558  -2.1946085  -1.6526893
Ep.1.3h    6.38516471   0.93659877   1.50802179  -0.3205125  -3.1942256
Mi.1.3h   -5.28265914  -5.88996623  -8.31051017 -10.2748142   0.7342194
Tr.1.3h    0.85823138   1.80524818  -0.03870608  -1.3618708  -4.1318739
Ve.1.3h   -7.29061870  -3.53579185   7.25601190  -3.0655909  -2.0452662
Da.1.24h  13.78606683 -18.40215240   6.60497063   6.9827022  -3.3200186
Do.1.24h  -0.91436016  -3.17539349  -4.01736353   1.0525898  -7.5326951
Ep.1.24h  16.44640381   8.32364323  -8.92134784 -15.8349740   2.8635797
Mi.1.24h  -1.45681885  12.12331550  -5.57858941  16.3782581  -0.7981441
Tr.1.24h  -1.69023803   2.32023552  -4.68892399  -0.6065746   7.3265560
Ve.1.24h -10.79282692   5.47202913   0.67211146  14.9278922   5.3081538
Da.2.3h   -1.91005140   7.71835432   5.99786233   0.4737518  15.0960743
Do.2.3h    4.96201006   8.09431107   3.87179158   0.9603996   4.0507437
Ep.2.3h    6.21814904   6.27757287   0.95842807   9.3494837   7.8643178
Mi.2.3h   -3.54034101   5.40153886 -20.09906718  -4.5593179   3.4521282
Tr.2.3h   15.01999636 -15.46336846  15.25022222   4.1638621  -8.7353334
Ve.2.3h   -2.65549417   6.13822990   2.85795777  -9.3132972  -6.5911907
Da.2.24h  11.32124512   9.91136366 -11.67499387   4.1363885  15.7547545
Do.2.24h  -2.47817364  -5.30552092   1.98407288  -0.5228760  -7.4662896
Ep.2.24h  -2.85410532  -0.38717032   2.34433748   0.6961883   0.5784496
Mi.2.24h -16.63925409  -1.24223415   4.05197392 -14.1265631  -9.7283645
Tr.2.24h -16.24544238  -0.22728360  -4.03336223   0.4888865 -10.8218935
Ve.2.24h  -0.16409120  -3.63248611  -8.93310319   6.0981233  -8.2253023
Da.3.3h   -8.26789568   5.08681164   3.29192204   2.2830477  -1.5613907
Do.3.3h   -9.11606652   6.32992727   3.81519442 -10.6191407   2.7229129
Ep.3.3h   -0.04361201   7.73429217   5.05020325   5.8327494  -7.1815933
Mi.3.3h   -4.13480031  -5.36598863  -8.98318427 -12.1418080   6.2099409
Tr.3.3h    5.06019751  -1.17004831  -2.52839193   0.5187929   3.8005108
Ve.3.3h    3.58676861   6.60162600   4.97661114   1.6629718  -1.5979081
Da.3.24h -14.64872344  -3.62865764  -4.34401466   5.7187557  -7.9362681
Do.3.24h   0.39438014  -4.93121619   6.67749017   8.9445941  -4.6016500
Ep.3.24h   3.68497326   3.81566091   1.83472811  -6.5627476   2.2829711
Mi.3.24h   9.06402224  -6.06188919   2.41187209  -4.6392254  -2.3740659
Tr.3.24h   2.09705908 -14.43947560  -5.41382058   6.0392405  10.4315736
Ve.3.24h   5.43568687 -12.80333188  -4.67890920  11.9986345   3.3023506
Da.4.3h    8.30469824  -6.30536048  -2.80653295  -0.3539695   3.7810573
Do.4.3h   10.80605449   0.05747227  -0.11839623   1.0260600  -2.6996732
Ep.4.3h    8.41486104  -1.76991337  -0.13873691  -3.3751075  -0.2199279
Mi.4.3h    4.46983721  -5.43977300 -12.41698889  -9.0080583   0.3874756
Tr.4.3h    7.32962483   0.47668734  -0.78669163  -0.3384197  -2.0229639
Ve.4.3h    1.95099080  -2.58829015  -1.88726819  -1.1294451  -4.3703533
Da.4.24h -15.23873771  -4.90959903   9.73936726   3.2448614   3.3723281
Do.4.24h -19.59309812   2.29708051   3.10827679  -0.3294521  -3.8654567
Ep.4.24h  -6.47510700  17.61719158   2.40867532   1.5181606  10.6130981
Mi.4.24h   0.45680913   9.90382700  -0.92057296   1.0114440  -5.6222415
Tr.4.24h  -4.51492966  -3.24626627  -1.37587562  -4.0003949  -3.8394723
Ve.4.24h  -2.64320429  -0.83084263   0.24589996  -3.5688127  -0.6695244
Da.5.3h   -3.01066882   1.89774697  -7.35390137   4.7184042 -12.2354758
Do.5.3h    9.84473706   9.47189679  -1.28516969   1.6926590 -11.8198304
Ep.5.3h   14.26029866   0.48979226   1.56316855   9.8726337 -14.5298436
Mi.5.3h   -1.96747237 -20.47747978  -8.46353713  -8.6975440   3.7494300
Tr.5.3h    1.46583556   6.81475135  10.25297695 -12.5189189  13.1866762
Ve.5.3h   -8.46083236   3.63941347  -8.08433206  11.6442545   8.7555749
Da.5.24h  12.25144472   3.64586842   1.72764737  -7.5187073  -7.2352899
Do.5.24h   3.70965855   4.74064147  10.99344897  -4.5166035   6.1469696
Ep.5.24h  -7.63764312   0.20219332   3.32761195   2.9470588   7.1047779
Mi.5.24h   8.36553616   1.29595685   9.89203896   9.6652037   4.9870850
Tr.5.24h  -3.00319106  -8.67762376  -1.23981290  -0.2884478   0.2152390
Ve.5.24h  -4.81089665 -14.85928909   2.02973816  -3.3884677   7.9790585
Da.6.3h   -4.13878012  -1.18598364   0.90802556  -0.9598823   0.8574955
Do.6.3h  -10.08948762  11.44777369   7.56503648  -7.2352492  -7.9995315
Ep.6.3h   -4.52807795  12.75794825  -1.13106712   5.6645804  -7.4280030
Mi.6.3h  -13.50493225  -8.65674910  -8.14286416   1.8417479   7.0497932
Tr.6.3h   -2.08914933   9.54811332   3.08152125   4.4513435   4.5822845
Ve.6.3h   -5.57024672  -0.68815234   8.55199071   8.2663902   0.5999482
Da.6.24h  -7.82452003 -14.22938387  -4.49903080  -5.7931538  -2.8169747
Do.6.24h   7.25252554  -1.87381356 -25.15933159   3.8336493  -2.5995267
Ep.6.24h   6.12019377   0.11407211  -7.68910644   7.5211865   5.6938413
Mi.6.24h  10.41084259  -2.38800274  12.74466664 -12.9682654   4.0459833
Tr.6.24h  12.84877558  17.84728096  -4.32846404 -10.1867181  -5.9221330
Ve.6.24h   3.85054048  -5.62825575  11.05271308   5.7331998   1.2475686
                  PC27          PC28        PC29        PC30        PC31
Da.1.3h   -4.681671895  -5.282165835   0.4751454   0.6369371   2.3055324
Do.1.3h   -3.636060820   4.747138238  -5.5988365   4.5600608   2.8057880
Ep.1.3h   -5.807194359   2.398714135   0.5207237   1.6382293   3.5308816
Mi.1.3h   -8.605955432   0.294584489  -0.4456016   3.0352939   1.3935090
Tr.1.3h    5.610482458   4.173156040   3.3879612  -4.4912450  -8.3307288
Ve.1.3h    1.153327515   0.627417724  -8.2994052   2.3173469  -3.5601678
Da.1.24h -14.630198462 -10.721305087   4.5852125   7.2278052  12.8855374
Do.1.24h   3.985143581  -0.002934811  -0.5409393   1.3653569  -9.7325225
Ep.1.24h   9.744082295   1.775564870  -4.7129836  -1.1751084   0.9733127
Mi.1.24h   8.189737394  -0.080733610   6.7099986  -9.1883044 -12.1723736
Tr.1.24h   4.995811815   7.109842933   6.0685750  -9.9776689   2.7576024
Ve.1.24h  -2.648740559  -3.634344216  -5.1693951  -0.2195497   7.5506093
Da.2.3h   -0.194410928 -11.036427381   7.0973780  -5.1656904 -10.1263645
Do.2.3h   -3.075766876   7.452271671  -7.1308887  -6.2661419  12.3544659
Ep.2.3h   -7.268023387  -1.294478804   1.1599269  -4.7074649   1.6677012
Mi.2.3h   -3.753180977  -5.394931218   5.5452383  -2.4687623   3.7612470
Tr.2.3h   13.486031868   2.949600479   1.5501864   8.9028731  -6.3269973
Ve.2.3h   -3.667309537 -11.335666273  -5.7057453 -10.1012804  -0.8079462
Da.2.24h  -1.985351656   1.640094759 -15.2744586  13.2075916 -10.6407145
Do.2.24h   1.660921853  -3.633469034  10.2200175  -7.3807647   3.5458457
Ep.2.24h  -0.856880131  -0.538882531  -0.5744913   0.6568753  -0.2894577
Mi.2.24h  -3.472332061  -6.706007510 -14.1631650   4.5360885  -0.5462716
Tr.2.24h   0.068783898  -1.396795788  11.4627590   5.6314383  -3.1006024
Ve.2.24h  -1.089577685  20.777769005   2.3018806   6.1042255   7.5554506
Da.3.3h    3.778386764  15.956077688  -3.3867822   5.6594654   6.9350786
Do.3.3h    7.057245430   5.273668463  -1.0735327   8.5343027   1.3912015
Ep.3.3h    6.821954766  -3.897012948  -0.7671128   1.4087992   2.0798803
Mi.3.3h    2.964339610  -8.693129470   5.7616911   2.1508677   3.0038210
Tr.3.3h   -3.676717210  -0.491186723   3.9630882   4.5013741  -7.7971289
Ve.3.3h   -1.956213708  -3.460261478   6.5716926  -2.8359399  -8.4442103
Da.3.24h  -1.963807930  -4.076340076   9.9539220  -1.0071593  -2.7986896
Do.3.24h  -0.193136363  -3.375965806   7.6775712   1.0539824   2.3268763
Ep.3.24h   1.933893322   3.351221882  -7.2404361   4.6334574   7.0992451
Mi.3.24h   0.585957484  -0.017490659  -9.3502024  -9.5138133   4.6024650
Tr.3.24h  -3.448678481  13.148435503   4.4256423  -4.4558745  -4.6762025
Ve.3.24h   0.673628124  -5.058101847 -16.6304071 -15.4431433  -0.5329054
Da.4.3h    3.731682362   3.682067323   3.7632711   3.2993807  -4.9443540
Do.4.3h   -2.634426297   1.769618424  -0.8252300   0.1755315   1.9465567
Ep.4.3h    6.835676467   2.311551165   3.2233531  -0.8705862  -1.4208476
Mi.4.3h   -3.761989544   2.040537047   5.8727837  -0.8276540  -0.8542813
Tr.4.3h   -3.423918432  -2.132157223   2.9948533  -1.9438679  -2.7442364
Ve.4.3h    2.294153087  -1.980692810   1.4123473  -2.2184597  -3.1613070
Da.4.24h  -9.100871117   6.849766180  -0.1461614  -3.9608260  -0.5666347
Do.4.24h  -3.014447707   7.258923683  -5.4040137  -4.6554132  -8.3625317
Ep.4.24h  15.082587453  -1.917832127  -1.9079119  -1.7680897   3.6327429
Mi.4.24h  -1.306143577  -4.825908730   1.1249295   4.1459272  -0.1313090
Tr.4.24h   0.004282254  -4.109368602  -0.3639432   3.6004151   5.8790355
Ve.4.24h  -1.322295907  -7.245164155  -5.9006782   3.9351748  11.1786451
Da.5.3h   -3.484119911   8.788612255 -11.5120431  -8.1781001  -3.6230164
Do.5.3h    1.438204128  -4.221002321   6.8892011   8.9788787   1.5178217
Ep.5.3h   -0.093266810  -2.458501613  -9.5559769  -5.6232532  -5.0999407
Mi.5.3h   -3.722409711  -6.260947050  -5.3631200   0.5587999  -8.4822073
Tr.5.3h   -8.162900489   2.770021318   8.0304412 -11.3633040   4.2846681
Ve.5.3h    3.057863521  -4.908983908  -1.2464356  15.9510049   0.8291806
Da.5.24h   1.630261395   0.518851669   2.7836058  -8.6371763   3.4155859
Do.5.24h  -3.413999882   2.489448906   2.2898005  -2.2501678  -3.8612210
Ep.5.24h -12.024032760   0.198629743  -3.9603009   9.2752654  -5.2059307
Mi.5.24h  -3.214110697   8.828095903   9.0282424   2.5414329  10.8035780
Tr.5.24h   9.921492189  -7.031409360   7.7188894   2.3222436   3.5965811
Ve.5.24h  15.036868996   3.264343419  -5.4710963  -2.8845927   1.2372235
Da.6.3h   10.040197830  10.338419235   2.8484835  -2.4940298   0.7260408
Do.6.3h    7.993195931  -1.845560492   0.2486814  -2.1833711   5.0919756
Ep.6.3h    0.421039681  -3.994345644  -2.0188270   1.4369426   4.5854799
Mi.6.3h   -0.094735065   2.268344679   3.1448088   4.1163319   2.1757283
Tr.6.3h  -11.031243272  -2.927802301   3.4038874  -6.8508191   1.1414144
Ve.6.3h   -3.574154355  -8.604119146  -5.7560367  -1.6263509  -3.3892613
Da.6.24h  -0.783984345   5.023340447   4.6395740  -5.2158376  -3.2747471
Do.6.24h  -4.111744917  -1.426776737  -2.6366651  -5.0777105   8.0229519
Ep.6.24h  17.759668783 -11.373095726  -0.9520102   4.9678736   5.0209136
Mi.6.24h   6.045283246  -0.609919252   0.6519764   3.6796269  -4.4461187
Tr.6.24h -17.380405566   5.198871091   3.8699678   9.4071279  -9.1609968
Ve.6.24h  -1.735776681   2.726217936  -4.2928744   6.8731922  -6.9999489
                PC32         PC33         PC34          PC35         PC36
Da.1.3h    0.9306414  -5.56457451  -4.07438100 -14.251764831   5.21456704
Do.1.3h   -0.8493857   3.78545061   0.88455564   0.866118782   2.99760042
Ep.1.3h   -3.7224851  -2.14971993   5.78342112  -4.789687821   3.01321997
Mi.1.3h    0.9682018  -0.79309631   2.70351629  -1.152796487   4.01798204
Tr.1.3h    5.9019661  -6.27920624   1.09431977   9.959182559  -6.18478156
Ve.1.3h    6.2579064   0.75596686   5.08025163  11.434519743   0.70225567
Da.1.24h   1.9747416   2.24989207  -9.11937213   7.177500035  -0.54234938
Do.1.24h  -0.9166763   6.19715120  13.88950354  -6.511680463  -3.30221459
Ep.1.24h  -4.1565253  -8.07620884   4.73496803   0.765475648   1.93433062
Mi.1.24h   0.4856429   9.07926808  -8.16930584  -3.215739312   9.98447395
Tr.1.24h  -3.1906994  -0.37109305  -7.92815566  -4.127060867 -12.35363812
Ve.1.24h  -1.7025730   1.52549777  -5.65698112   0.082146861  -3.65673655
Da.2.3h    4.4858284  -2.08862366  10.70173583  10.019569868  -0.74336592
Do.2.3h   -0.8104843  -0.49047990  -1.79998821   0.273851315  -1.42975928
Ep.2.3h    4.6953652   8.97260152   3.54663033   3.346628433  -5.32642308
Mi.2.3h   -2.4449196  -7.19265247  -0.58344071  -1.950957494  -3.34546946
Tr.2.3h  -17.2412438  -3.08218172  -1.76395067  -8.034478975   3.13860256
Ve.2.3h    3.2967363 -11.73726903  -8.37000659 -10.307008870   5.78442419
Da.2.24h   2.3082565   7.34334289   6.18648161 -10.998076957  -1.58825621
Do.2.24h  -4.8857932  -5.40979486   0.19821409   6.669413234   2.81383886
Ep.2.24h   0.3538267   1.82137510   0.02071197  -0.604126563  -0.87928632
Mi.2.24h   2.0471484   4.30065095  -6.38473822   3.109999873  -8.52174902
Tr.2.24h   4.5071863   8.26843080 -10.78551911  -1.554700424  -0.99031576
Ve.2.24h   8.1814867  -0.17979578   8.50863210   6.063512883  10.68828838
Da.3.3h    5.8232327  -3.33485645   2.31420722   0.841217846   0.03035415
Do.3.3h   -3.1539690   7.55819819  -2.51383985   0.583065994   1.55430265
Ep.3.3h   -1.5696030   2.70807919   1.50523306  -2.878863142  -2.91203636
Mi.3.3h   -1.3451468   0.56434610   1.49743078  -3.154830560  -3.49136829
Tr.3.3h    2.3540169   2.46006522  -0.78798340  -0.878535717   2.58208954
Ve.3.3h    4.9507254  -5.14145464  -0.94206180  -3.469390641  -0.08743440
Da.3.24h -11.1521719  -3.47644911   6.47912085   7.526126041   8.80453589
Do.3.24h   1.4032846   0.75955242   3.54010647  -8.065269492 -10.63175492
Ep.3.24h   9.2807485   7.51572091 -13.34437099   2.649866392   7.15203930
Mi.3.24h   0.2815304  -6.85656089   9.42679596   6.881637638  -1.88658114
Tr.3.24h  -6.7632093  -0.71005268  -5.61677828  -3.349237923  -3.40452074
Ve.3.24h  -6.2425964   1.78168498   1.26812157   7.834065452   6.12294057
Da.4.3h   -0.4970756  -2.25794636  -5.22157014   1.004488932   2.51492804
Do.4.3h    0.9502061   2.40807734  -3.45472892  -0.641806878   0.97565448
Ep.4.3h   -0.8338176  -0.01389859  -2.63123405   2.768312182  -1.01812361
Mi.4.3h    2.6687010   1.43149304  -5.40924837   0.862563395  -0.61088635
Tr.4.3h    3.4405922   1.66657066  -0.04219099   0.002193181   0.01620168
Ve.4.3h    4.9010808  -0.03328198   1.40667613   3.241606803  -3.12653993
Da.4.24h   0.6937997  -1.69468576  -3.05931052  -1.182139163  -2.04149852
Do.4.24h   4.1945231   2.24960289   4.21924398  -6.330105655  -4.28081446
Ep.4.24h   0.1179870  -9.28971769  -7.78180486   6.448011811  -1.58738651
Mi.4.24h  -6.3095654   3.03951434   5.62372139  -1.121797672  10.16472926
Tr.4.24h  -6.5321298   2.04165116   4.86796457  -2.044117746  -4.88937088
Ve.4.24h  -6.0704929  -1.86028854  10.30206809  -2.950067242   1.43658774
Da.5.3h   -0.5841446 -16.17776785  -6.27654779  -5.113374514   5.50933902
Do.5.3h   14.0527761  -3.38911243   3.30896085   2.496903073   0.48792090
Ep.5.3h   -3.8593962   9.98025901  -2.80849435   4.655067090  -7.82216760
Mi.5.3h   -2.8815205   6.28335135  -5.03378163   4.047670660  -2.25107916
Tr.5.3h   -7.6182845  15.04452010   5.44066159   1.627604843  12.85645943
Ve.5.3h   -9.0137166 -10.02515831   1.04481877   4.961721425  -5.04481873
Da.5.24h   7.0679902   6.37580029   0.20050013  -4.447709526   1.85630807
Do.5.24h  -7.5874404  -0.68006026  -0.85330026   8.370861063  -9.67440651
Ep.5.24h  -5.3321423  -6.23968276  -3.09201045  -1.920332477   9.52446351
Mi.5.24h   1.6105609  -4.83337758   1.56647970  -1.791168044  -7.22153957
Tr.5.24h   8.8276865  -0.92221050   6.16988532  -8.014820494   0.45245456
Ve.5.24h   7.5276438   3.11776445  -0.39259172  -2.939073018   1.39695906
Da.6.3h   -7.5707837   0.16811186   2.00385478  -0.955037167  -1.65112627
Do.6.3h  -10.8830156   6.56970106  -0.97936340  -1.978348110  -3.30771164
Ep.6.3h   -4.5375145   7.16317679   0.82515951  -0.405808874   1.17637154
Mi.6.3h    2.9616300   1.61611755   0.82016840   4.724166839   0.51034681
Tr.6.3h    4.9313861  -2.57709185   2.71398140  -6.621501197   2.33127727
Ve.6.3h    7.9412356  -6.24227813   4.48381854  -0.790481950   0.42016380
Da.6.24h   3.5669271  -2.15280957   1.07197051   1.045991186   2.44167063
Do.6.24h  -0.5960328   2.93669813   2.92418885  -6.286896651  -7.80510245
Ep.6.24h   2.8349318   0.43336992  -7.92405167   4.872986321   9.40714331
Mi.6.24h   5.3493929  -4.63320327   0.64214604  -1.689559227  -2.51694824
Tr.6.24h  -7.3929889  -4.63114291  -6.82400828   6.360295823  -2.74178460
Ve.6.24h   4.1200200   0.41472964   0.62488459   2.944008920  -1.14547881
                 PC37          PC38         PC39         PC40          PC41
Da.1.3h    5.28105146   1.341398824   1.23513980  -9.35791896  -3.643339318
Do.1.3h    0.91086274   2.001903814  -2.67899704   1.01490401   2.364475203
Ep.1.3h    5.04731851   5.580897431  -0.04291028   4.44028786  -1.889790268
Mi.1.3h    1.55406483   1.789369875   0.72941746   1.99585440  -1.471918891
Tr.1.3h   -9.25749611  -0.336624851  -3.29650310   2.30309044  -0.099324600
Ve.1.3h   -7.64338594  -3.541585917  -5.83454050   1.36306396   5.887492415
Da.1.24h  -5.12515369  -5.257196397  -2.26828290   8.63847969  -5.546881675
Do.1.24h   0.00648092   5.218779313   5.80790659  -7.08866027   2.673610274
Ep.1.24h   2.97872584   2.377144297  -3.05200820  -1.98881617   3.637048783
Mi.1.24h   6.68630059  -7.972227451  -1.31947232  -1.24985351  -4.251992062
Tr.1.24h   3.94203473   1.352071648   3.57209469   3.42163304   0.144362806
Ve.1.24h  -1.90369651  -1.698481419   8.16040213  -2.80561801   0.761490131
Da.2.3h   -7.37841741  -8.542645528   2.19014592   6.44120396   5.834617304
Do.2.3h    4.34396773   7.922756344 -11.48925468  -7.56266888  -4.060222432
Ep.2.3h    2.74291101   4.175212338  -1.52837589   0.67849294  -2.493721821
Mi.2.3h   -0.84932829   6.002796843  -1.02202101   2.32983360  -3.462778067
Tr.2.3h  -10.47292136  -4.896257477   7.74411428  -1.39953775  -3.172615254
Ve.2.3h    7.83630228  -6.053006320   1.32388893  -0.63352071   6.762236618
Da.2.24h  -2.95017987   0.047340810  -0.99480331   0.80611939   1.627026882
Do.2.24h   2.70805751  -0.082551176  -0.62762454  -0.65054192  -1.847392842
Ep.2.24h   0.52921155   0.335364217  -0.55068671  -0.62122332   0.244348160
Mi.2.24h  -5.17964553   4.778600422   6.54493423  -6.15563410  -1.944962000
Tr.2.24h  -0.04419422   3.027069544  -1.58822435   1.31299777   2.638478484
Ve.2.24h   8.56165558  -5.318541976   1.23592522   5.90619847  -0.976228656
Da.3.3h   -4.68389079  -0.729700365   3.84794146  -3.75230753  -0.102492843
Do.3.3h    3.00478294  -0.068775400   3.62300158   0.92677265  -5.020652419
Ep.3.3h   -1.73264634  -6.197099512  -8.17577116  -4.15426503  -0.411697924
Mi.3.3h    2.57560566  -6.814019115  -2.73187173   2.07732416   7.614134775
Tr.3.3h   -1.10217111   1.682461862   4.89868189   2.01208940  -5.326647311
Ve.3.3h    3.72729336   6.732607609   4.23657466   8.30814121  -2.860217729
Da.3.24h   0.40319247   3.073043184  -5.33334128 -12.98466511  -2.191647782
Do.3.24h  -0.34873106   5.647890372   5.17308484   8.81158801   0.243244213
Ep.3.24h  -1.68678739  -8.299197827  -0.96385356   0.86464842   2.110193012
Mi.3.24h  -2.92475691   5.647368323   1.79459404   1.80096459 -10.022474429
Tr.3.24h  -6.53267541  -0.142610876 -16.90661406  -6.84666529   7.392020147
Ve.3.24h   7.81540337  -0.410544981   7.35624919   1.68965765   7.183204970
Da.4.3h    0.70525663   0.675263909   7.88181420  -0.89206416  -2.059182352
Do.4.3h    1.23475088   0.831026440  -3.38953035  -3.57709430   1.496506310
Ep.4.3h    0.04168979  -1.348078469  -0.87650688  -1.87311789   0.005550194
Mi.4.3h    0.99051992   2.377153339   3.14596030  -0.75381822  -0.324736356
Tr.4.3h   -2.85598752   1.127290809  -1.13252813   0.51536399   0.144753992
Ve.4.3h   -4.34559378  -0.608725113  -3.05064531   1.36640467  -0.429612270
Da.4.24h   1.11586354   2.586506014  -1.37963099   3.52102046   2.904713058
Do.4.24h   1.32406768   3.411451512   0.04115751   3.91830581   0.508070569
Ep.4.24h  -4.65177229  -3.246620434   2.15285393  -5.67914106  -4.668487304
Mi.4.24h   1.24847256  -3.538859966  -5.25290092   4.52515339  -3.670254509
Tr.4.24h   0.88271528   0.249431241  -2.59853067  -0.04518158   1.876061391
Ve.4.24h   2.69816104  -2.211211743   2.56413592   1.09113055   4.633608251
Da.5.3h  -10.62450258  -1.374851502   2.88889854   5.05603588  -0.697705191
Do.5.3h    7.00511079  -3.367365737   5.45775405  -6.86086157   3.656426794
Ep.5.3h    6.02008904   6.455759401  -5.53001209   2.41195182   0.859952884
Mi.5.3h    1.50218291  -3.596265457   0.29969423  -2.19996929   3.136999339
Tr.5.3h   -9.46650005   2.151900196   7.97846340  -2.99129694   0.468923567
Ve.5.3h    7.79095518  -2.447348833  -0.56442927   1.16580928  -0.065413110
Da.5.24h  -3.96906670   0.677796447  -0.67458198  -2.81139923   1.502414252
Do.5.24h  11.47693332  -5.304672822   1.70029622  -1.79902072  -9.443769933
Ep.5.24h  -2.81025350   1.157773933  -7.03396248   9.68542184   0.020829070
Mi.5.24h  -2.67899091  -0.253049025   6.30136625  -3.24597613  16.798466295
Tr.5.24h  -6.34580865   3.749438059  -9.08638854  -3.44796673  -6.120042908
Ve.5.24h   1.94087010   0.116044898   0.19445617   4.42186281  -9.318269074
Da.6.3h    0.57119220  -0.014666070   1.15504211   9.47992462   0.532660910
Do.6.3h   -0.02539650  -3.122919235  -2.67376318   6.36128516   3.576048308
Ep.6.3h   -2.08207952  -1.694574430  -2.47635101   1.98082184   2.152265726
Mi.6.3h    1.35507342   3.339893994   3.08763480  -3.20915695  -6.864415485
Tr.6.3h   -2.50200323  -0.007009004  -0.34945838  -0.31929708  -3.203233441
Ve.6.3h   -2.55193416  -0.833420787  -1.62479200  -5.65184531  -2.797548883
Da.6.24h   6.51816413  -2.236895905   3.43215567  -3.05759395   8.203321826
Do.6.24h  -7.39122829 -15.663786986   3.36856796  -2.54247952  -6.883697428
Ep.6.24h  -3.62198715  20.432539940   1.55977479   2.82934892   7.726686371
Mi.6.24h   8.02082847  -3.651836370  -9.33038582   5.84883020   0.088692026
Tr.6.24h  -3.35604507   4.963552632   2.85028271  -3.61687678  -0.171906390
Ve.6.24h   5.99710791  -2.153675359   1.89514897  -9.49596291   0.104337648
                 PC42        PC43         PC44         PC45        PC46
Da.1.3h   -0.10582757 -6.24562170   3.10624765   3.59539885  0.45886826
Do.1.3h   -0.34912201  0.17100001  -0.59540194  -4.84457823 -4.80810669
Ep.1.3h   -3.92723914  0.21062126  -0.04805682   2.10673156 -0.68572945
Mi.1.3h   -5.89656198  2.06423461   2.94329931   1.81840647 -4.25040082
Tr.1.3h    1.77902451  3.32863061  -2.14064319   5.48750252 -0.10860546
Ve.1.3h   -1.70256272 -2.37473599  -3.26625495  -6.81423232  8.27730103
Da.1.24h  -0.29007073 -0.23622330   0.57162448   2.91261524  1.67432048
Do.1.24h  -3.68405707  1.42881605   0.41456855   5.04385165  1.88154825
Ep.1.24h   1.39520522 -3.22558070  -3.14480232  -9.15175716 -3.03771805
Mi.1.24h  -3.36329982 -1.54595764   5.79639054  -0.66266493  0.32995948
Tr.1.24h   7.12233752  2.60311970  -2.04631689   5.37444004  1.51265248
Ve.1.24h   7.19141250  3.10266758  -0.77080747  -6.32581071 -2.62214443
Da.2.3h   -1.27457748 -4.94669242   6.79253568   5.14760215 -1.88602148
Do.2.3h    1.58565847 -0.97706458  -0.57737965   0.96042329  5.71558521
Ep.2.3h   -1.69029898  3.22799354  -6.74663077 -10.74948804 -4.17336789
Mi.2.3h   -4.44265068  5.56515758  -3.15270629   6.42006682 -3.24796588
Tr.2.3h   -0.05135975  5.98732644   2.05044609  -0.95540639 -2.38988665
Ve.2.3h    2.20296355 -6.58102019   4.30222254   1.16848911  8.70419805
Da.2.24h   1.14414239 -0.03120077  -0.31685778   1.28835494  1.83751436
Do.2.24h   1.01521878  0.01637179   0.15151892  -0.05758551 -0.82038802
Ep.2.24h  -0.32892265  0.45026138   0.13958830   0.04659657 -0.62387225
Mi.2.24h   2.62011291  4.54050070   0.69930223  -4.02890083 -2.16022002
Tr.2.24h  -6.06815760 -1.39981031  -7.19202379  -1.35994827  3.74756932
Ve.2.24h   7.57045214 -4.80889692   4.86456257   0.92369602 -5.24999881
Da.3.3h   -4.25479681 -2.47168569   3.53895408  -0.18003649  4.24058583
Do.3.3h   -1.59302155 -8.95955787  -5.51622125   3.92191409 -7.97120985
Ep.3.3h   -1.79092355  3.25122042   1.46051515   3.79897653  1.50562334
Mi.3.3h    7.82948698  3.68477721   0.22649823  -2.34220140 -0.72569589
Tr.3.3h   -1.53254947 -7.41797295  -1.99931962  -4.72001762  4.71383424
Ve.3.3h    3.40852209  2.78950913   1.83462908  -5.49794586 -1.27709094
Da.3.24h  10.10907331 -5.19505459  -7.52101883   2.06216268 -1.79572388
Do.3.24h  -1.49998188 -3.11235127  -1.80834757  -4.34624048 -2.01738277
Ep.3.24h  -4.87754525  4.09275452   3.63249338   4.04363613  3.38214440
Mi.3.24h   2.00149181  3.52740913  10.20214186   2.19205151  0.59863877
Tr.3.24h  -4.00973963  5.15880770   7.06932916  -0.24404444 -2.23657423
Ve.3.24h  -4.15174949  4.81108649  -9.74468438   3.47625034  2.00958232
Da.4.3h    8.38043706  4.84425176   0.03085585  -6.90643457  8.96974935
Do.4.3h    0.79450461  2.50316535   0.39971153  -2.36615383 -5.10719861
Ep.4.3h    4.26522783  1.60315463   1.20137241  -0.22660319 -0.51420947
Mi.4.3h   -4.01931435  2.51138387   1.69525978   4.95380467 -5.54935999
Tr.4.3h   -3.00898929 -2.74028297  -1.37328991  -0.85159185 -3.15724584
Ve.4.3h   -2.54516249 -7.25572212  -0.34231817   2.87531892 -1.56980426
Da.4.24h   4.20665236  0.69279743  -1.78476233   2.20626817 -0.60430501
Do.4.24h   4.11854417  3.84955865   4.12704087   6.82360902  2.52435396
Ep.4.24h   1.45321234 -1.95143050  -1.70491820  -4.03696303 -3.75826631
Mi.4.24h  -4.85383401 -0.33466421  -4.05379820  -2.82476639  2.05871783
Tr.4.24h  -5.07010629 -2.20358656  -0.30675081   4.44827240  6.11208702
Ve.4.24h  -2.13650355 -1.46603512   2.65519137  -2.34625333  0.94212576
Da.5.3h   -9.64878109 -0.57065438  -9.44771893  -0.65746840 -3.15203856
Do.5.3h    4.48173184 12.89263177  -0.22639316   5.69076903  1.52383485
Ep.5.3h    6.17895457 -6.41845982   6.10308364  -1.42610989  1.75735979
Mi.5.3h    3.98812153 -8.33104085   0.06774726  -1.28293643 -3.30257918
Tr.5.3h    1.72197601  5.85445694  -1.91576496   2.30597664  3.23106076
Ve.5.3h   -2.41300218  2.23301857   3.90044654  -1.09677150  0.07292879
Da.5.24h   2.97778055 -2.53719978  -1.98603431  -0.64487540 -0.43710580
Do.5.24h -15.60160621  0.89754343   2.65117383  -0.01870588  2.97371723
Ep.5.24h   9.23212632  4.60894855   0.13889856   1.58118416 -0.26380899
Mi.5.24h  -2.11963895 -8.51524267  -2.39624019   0.76929736 -0.96787654
Tr.5.24h  -2.36369379  0.64148128   0.03302582  -5.96563385  4.34307449
Ve.5.24h   2.95477022 -2.86734702   2.46410627   0.14478344 -6.83429913
Da.6.3h    2.81928250 -2.69846004  -2.85733450  -0.14640113 12.04032943
Do.6.3h    0.64903875 -1.32539022   4.98201504   1.76109083 -7.41475045
Ep.6.3h   -0.45113518  8.35707601   1.66614406  -2.50731886 -1.80764187
Mi.6.3h   -1.66975522  0.02246397  -0.31753015  -0.81968624 10.29243152
Tr.6.3h   -0.20320618 -0.36191138  -0.28327698  -3.21359856 -4.41019521
Ve.6.3h    0.67766370 -1.13943283   3.13106321   0.15205789 -2.70067574
Da.6.24h  -7.81842260  5.47229412   7.13397674 -12.86207694 -2.89291136
Do.6.24h   4.08624000 -4.11611663  -3.10874113   1.76972749  1.70573417
Ep.6.24h  -3.59307909 -2.63931212   3.85039187   6.14035129 -0.13807792
Mi.6.24h   1.85751443  5.29713824 -13.86681464   1.53061747  0.34810773
Tr.6.24h   2.60516230 -5.21755796   4.93382736  -0.51606669  2.56934644
Ve.6.24h  -0.02279701 -0.07435637  -8.40303971   8.05497928 -5.38443123
                PC47         PC48          PC49         PC50        PC51
Da.1.3h   6.64629557   2.33570569   1.336797654   1.11992329 -3.55203678
Do.1.3h   0.40940222  -2.71976212   3.919397284   4.09475414  1.40802263
Ep.1.3h  -3.49059667   2.88640662  -3.291898593   1.59379619 -0.04676964
Mi.1.3h  -2.58958952  -3.38195959  -4.927283788   0.26623243  4.25838545
Tr.1.3h   3.27832674   5.05671135  -2.376703000  -4.48409641 -4.67153186
Ve.1.3h   0.87868791  -1.80870186   2.766224283   2.38063673  2.08783280
Da.1.24h  0.13750718   0.30932512  -1.966219019   1.13830129 -0.94669882
Do.1.24h  2.67187104  -2.52791992   8.370728616   1.12604270 -7.86205996
Ep.1.24h -3.27528454   3.10673884  -5.313725601  -1.58466872  8.59094400
Mi.1.24h  1.46033054  -0.32333494  -5.481342036  -0.49538742  0.89168698
Tr.1.24h -0.75159804  -1.07709979   0.980614793  -2.62209172  3.22032309
Ve.1.24h -4.69806336  -0.58961510   5.228147488  -2.26233589 -2.78791450
Da.2.3h  -5.14073789  -5.36608152  -0.421938096   3.03913079  3.85599157
Do.2.3h  -1.69096284  -8.02993509  -0.329636805   1.14465031 -1.17426177
Ep.2.3h   1.02772594   8.09886007   5.235333369  -4.22013158  1.56489880
Mi.2.3h   4.58395505  -0.41722661  -4.140166017   4.96853453 -9.08259627
Tr.2.3h  -1.75412434  -0.02291070   0.750687116   0.20226322  2.27513093
Ve.2.3h  -1.56402693   5.65647880   0.840567305  -3.84636623  4.04421193
Da.2.24h  1.92774328   0.03488489   1.008070338   0.11520100  1.73044867
Do.2.24h -0.67228168  -0.15816405  -0.037742116   0.22691578 -0.49198292
Ep.2.24h  0.10653351  -0.13347931   0.063168277  -0.07737358 -0.12076381
Mi.2.24h -6.69929643   0.88024782 -10.489005789   0.75529400 -4.95329535
Tr.2.24h  9.68248101   1.47129870   7.704833567   3.56252378  9.50165489
Ve.2.24h  0.53092903  -1.70483551  -0.916628710  -5.81269898 -6.79329235
Da.3.3h   4.27839544  12.22964055  -6.188316319   9.59992777  2.91056238
Do.3.3h   4.52756233  -7.06122647   1.027447198  -3.01493477 -0.78304234
Ep.3.3h  -6.50172763  -8.13276791  -4.415797104 -13.25102448  2.83527486
Mi.3.3h   0.86965684   7.94606770   5.035960770  -5.81673824 -4.14707307
Tr.3.3h  -2.88805232   3.40563069  -2.314101551  -6.85765152 -5.51851719
Ve.3.3h  -7.96892183 -10.87938217   5.035937420  14.18226818 -0.12546230
Da.3.24h -5.61870769   2.81117359   3.262490669   3.59007746 -0.67061051
Do.3.24h  4.03784320   0.16647975 -10.667796050  -3.55335225  4.69291896
Ep.3.24h -2.04995548  -1.83774057   6.367344987   1.95042040 -5.82023293
Mi.3.24h  8.25492172   1.44687193  12.320603705  -4.14798732  8.46652634
Tr.3.24h -2.65606000   2.58055930  -2.078056073   5.76365628 -0.27703201
Ve.3.24h  6.12468187  -2.34009405  -5.153753378   2.24134545 -1.70742990
Da.4.3h   2.70393047  -1.10354559   1.335023632  -0.33422086 -4.78033110
Do.4.3h  -0.31129640  -2.49638803   1.996583786  -0.76139486  2.22746022
Ep.4.3h   1.46979857  -3.26069712  -0.486219136  -1.37005457  0.06900698
Mi.4.3h  -1.04453529   0.99067181  -0.245100910  -0.21877404  6.35806981
Tr.4.3h  -0.91738321   1.94547079   0.918099031  -0.33072415 -0.26734577
Ve.4.3h   2.67613680   2.92402404  -1.776529535  -1.46359215 -3.86125159
Da.4.24h -3.94095088   3.48382243   2.363315174  -2.50816315  2.61072294
Do.4.24h -2.52360759   0.33901249   0.517815754  -2.16646016 -1.16445219
Ep.4.24h  7.86366550  -3.60031615  -3.480170831   3.35724467 -0.17618506
Mi.4.24h -2.98354907   4.77818393  -0.273904243   3.51047171 -0.37428878
Tr.4.24h -1.55215671  -3.99492325  -2.490066252  -0.24581108  1.49824400
Ve.4.24h -0.25370842  -1.17558449   0.930530386   3.36902188 -1.04755988
Da.5.3h  -3.65804568  -2.62692073   5.558138563  -5.22163601 -1.10459698
Do.5.3h   0.05495726   1.73089696  -2.275461653   4.81415958  3.99939451
Ep.5.3h   4.31652132  -0.44551362  -4.021587519   2.58683989 -1.73456513
Mi.5.3h  -1.06212565  -1.00367617   1.975124541   1.78681864 -0.05536315
Tr.5.3h   3.22670071   1.07992104  -4.437667791  -2.83492634  0.15407368
Ve.5.3h   2.03548210   1.41989672   1.965640149  -1.95920592  0.79413055
Da.5.24h -0.90454658  -0.76102690   0.020376238   1.55571375  1.65237210
Do.5.24h -4.56541435   4.76237797   4.169489771  -0.77521397 -4.08104654
Ep.5.24h  7.90180027  -2.50571083  -3.990372734  -1.19006846  3.68865546
Mi.5.24h -2.37023714  -3.41790896   1.436758793  -0.15543901 -2.77875108
Tr.5.24h  3.18608851  -4.07318878   1.220733621  -3.57777530 -3.48730266
Ve.5.24h -8.96845656   6.23286431  -0.424586179   4.54575977  1.12737159
Da.6.3h  -2.66673129   3.21214920  -1.346519680   4.17651333 -1.60356067
Do.6.3h   7.17534589  -0.81767447   3.522799717   2.44747861 -0.67572909
Ep.6.3h  -5.28709433   5.68814308   0.971378023  -1.70398777 -0.39926897
Mi.6.3h  -4.11633000  -7.41722579  -1.802050194  -3.32615536  8.03785301
Tr.6.3h  -0.57999439   0.90908871   1.224930308  -0.47100339 -1.44448876
Ve.6.3h   4.40305748   1.24557313  -3.886653959   1.25021920 -3.92459607
Da.6.24h  4.83975261  -5.67191230  -3.014995785  -3.27502449 -1.70797408
Do.6.24h -0.33206319  -1.55220785   0.861250415   5.47303641  2.37476594
Ep.6.24h -5.30063229   3.51646755   2.432567777  -3.15702597 -1.31593075
Mi.6.24h  0.36829067  -2.00846942  -0.742378666   1.01610989 -5.36215933
Tr.6.24h  0.72939309  -0.48733262   0.538518618  -2.21728615 -1.95541342
Ve.6.24h -1.03692543   2.25081473  -0.009054026  -1.64050078  7.90783024
               PC52        PC53        PC54         PC55          PC56
Da.1.3h  -6.8780193 -8.00516922 -0.39838458  -1.04864917  -0.514600698
Do.1.3h  -0.1818470  3.07594948 -3.14842431   0.04172312   4.759478225
Ep.1.3h   4.7516591 -0.16527150  1.20834916   1.33770633   2.555809789
Mi.1.3h   1.9732429  7.68037372  2.93973776  -0.50282055  -4.433737443
Tr.1.3h   0.7669524 -5.76182996 -9.60187112  12.74517928   8.225724887
Ve.1.3h   0.7909570 -0.98214301  0.16274332  -9.15053776 -10.610382858
Da.1.24h  1.8294034 -0.40607418 -0.76146308  -0.62859385  -0.480781117
Do.1.24h  3.4363849 -5.11282702  7.39763752   2.72098853  -0.789465055
Ep.1.24h -4.3452044  4.18840190 -4.56344562  -1.70562529   1.702294165
Mi.1.24h -1.7453633  1.39802034 -5.76347379  -2.19486644  -2.263046233
Tr.1.24h -3.0217787 -0.92456839  6.75173708  -0.22693442   1.404922363
Ve.1.24h  1.1471487  5.03347811  6.61423878  -2.01482196   0.884813845
Da.2.3h  -3.1675920  1.53108226  3.16746246  -2.62866050   5.899505275
Do.2.3h  -3.7251551 -2.82103755 -2.51715862  -3.09175818   9.373105479
Ep.2.3h  -0.1992500 -3.95920620 -1.26399285   8.43715489 -11.398138213
Mi.2.3h   7.8850652  1.87556003 -2.55612448  -5.50932755  -4.663586478
Tr.2.3h  -5.5265924  1.96722496  1.21168081  -0.49422065   0.001851310
Ve.2.3h   8.6428963  2.22401734  3.14544987   5.80790854  -2.201841433
Da.2.24h -0.2743380 -0.59487973 -0.09330981   0.47044833  -0.002537996
Do.2.24h  0.1217745  0.40637424  0.02967994  -0.02360910  -0.400725098
Ep.2.24h -0.1925718  0.25682954  0.06625155   0.07137899   0.049708291
Mi.2.24h -3.2678966 -1.47570033 -0.97123657  -0.20363537   0.542127721
Tr.2.24h -0.4559112  0.23163768 -2.00324859  -2.98735679   3.677495067
Ve.2.24h -0.5154090 -0.04898404  1.35054041   0.57535882  -1.625372093
Da.3.3h  -0.5219122  6.35081251  5.90635243   4.96632927   1.587622536
Do.3.3h   0.4082138  1.84249600 -2.51034930   0.40131096   1.750583453
Ep.3.3h   3.2386596 -2.23238199  1.58192069   3.14833714  -6.517952446
Mi.3.3h  -3.9551904 -3.98391892 -4.83722731 -10.30025143   3.837287463
Tr.3.3h   2.3076499 -0.09047194  1.07539100  -2.25370044   1.275850469
Ve.3.3h   3.2686981 -2.08479523 -3.77485374   3.35103761  -0.015700623
Da.3.24h -0.8192321  1.55050170  2.45395044  -0.12853822  -2.605694445
Do.3.24h  0.6560134 -1.89908899 -1.35796588  -1.18154071   0.750543325
Ep.3.24h -0.4048026 -0.24857145  0.17548966   1.65973362   0.793453718
Mi.3.24h  3.2376199  0.01436570  1.40629996  -1.82028917   0.544134467
Tr.3.24h  2.1271197 -2.24751243 -0.62101484   0.81772561  -3.397200462
Ve.3.24h -6.7554753  2.23880301  0.86542951   2.20823523   0.864490146
Da.4.3h   3.9479872  5.32423564 -6.15802391  -0.75711329   0.112797592
Do.4.3h  -2.0900357  2.91071733  0.71105465  -1.16317885   0.634552153
Ep.4.3h   4.6366151  3.53002844 -2.06366273   1.79706056  -2.032925471
Mi.4.3h  -1.0293772  0.59376906  5.88489045   0.02470292  -1.860900782
Tr.4.3h  -4.2221886 -0.75479914  5.08227616  -0.90815046   2.302597368
Ve.4.3h  -4.5212579 -1.52025676  5.70949325  -4.36313578   0.965054111
Da.4.24h -4.1337015  3.74539829 -2.80234647   3.11442868   0.605481889
Do.4.24h -2.7008776  6.41907996 -4.92238225  -3.19018496  -1.429548506
Ep.4.24h  6.2094248 -6.30622517  5.71127899  -0.06208719  -1.106816743
Mi.4.24h -1.1968162 -4.82522646  0.85581644   2.58946241   5.288145983
Tr.4.24h  0.5963298 -3.15204635 -1.22834237  -0.72000323  -0.891234739
Ve.4.24h  1.5581338 -4.85724990 -7.58841111   3.91504412  -2.122962992
Da.5.3h  -2.0906929 -3.43812387 -2.07581289  -3.68566497  -0.522984453
Do.5.3h  -7.2913185 -3.63923495 -0.97035190  -1.30584248  -2.715822673
Ep.5.3h   1.5256205  0.36465015  3.36147596  -4.98319107   1.242482099
Mi.5.3h   2.5451019  1.59492228  1.06485912  10.45128528   3.214705728
Tr.5.3h   3.1681610  0.36935075 -1.08488594  -1.49688663  -0.826253952
Ve.5.3h  -2.3680105  1.17221869  0.79747956   1.29782607   1.533881038
Da.5.24h -0.2805207 -1.58953278  0.46357035   1.01799485   0.154582416
Do.5.24h -1.3206720  4.77961932 -2.54503418  -1.80976242  -0.102641401
Ep.5.24h  1.6168520 -3.73530072  3.07706225   0.92030265  -0.121545205
Mi.5.24h  2.6457722  0.95757559 -2.37917323  -0.00977928  -1.116438882
Tr.5.24h  0.2359172 11.91087733  0.85599632   2.93776379   3.564783751
Ve.5.24h  2.0357209 -8.88104012 -0.60748362  -3.71368249  -3.705796379
Da.6.3h   2.3951696 -2.00615189  1.34969230  -2.65259384  -1.421652516
Do.6.3h  -4.2627099  1.95495016 -3.86631248   4.34140349  -6.798546998
Ep.6.3h   7.6037653 -1.53440470  5.52433394  -0.88315796   9.595086192
Mi.6.3h  -5.8105771 -6.01077304  0.16365024   4.81793084  -0.621862378
Tr.6.3h  -5.1214341  1.46363872  1.65516620  -0.56330406   2.001240277
Ve.6.3h  -1.2840556  4.88371392 -4.73773394  -1.37367140  -2.130017336
Da.6.24h  2.5895984 -5.60544415  2.71279706  -1.57229617   4.519676056
Do.6.24h -0.6647211  2.62212383 -3.89392075   0.73263138  -0.076961937
Ep.6.24h -1.6270984  2.57375116 -2.32550431   0.56495242  -2.049606076
Mi.6.24h -1.9628549  4.26151418  8.82357725   2.49363092  -2.594229328
Tr.6.24h -3.6723490 -2.51340893  0.64157250  -0.20603769  -3.891339810
Ve.6.24h 13.7051821  0.11558764 -5.96345879  -6.26151486   3.844982602
               PC57       PC58          PC59        PC60         PC61
Da.1.3h  -5.0176582 -3.5661348  8.336996e+00  5.12157066   3.08464028
Do.1.3h   0.5128667  1.1612208 -9.343601e+00 -6.02282375   6.15525053
Ep.1.3h   4.9395848  2.4802122 -7.232061e+00 -1.62279204   2.83660561
Mi.1.3h   0.7172074  2.7836297 -1.779731e-01 -1.55149795 -11.74354627
Tr.1.3h  -4.5846444  1.6741397  3.410919e+00 -2.29814499  -1.71494823
Ve.1.3h   0.2587333 -0.6758582  5.967159e+00  4.97225366   0.06217548
Da.1.24h  2.1157145 -0.4101931 -3.727152e-01 -0.62595143   1.62574656
Do.1.24h  2.4311156  3.5343658 -1.155852e+00 -2.95052577  -3.79245216
Ep.1.24h -3.9879027 -3.8468701  1.518554e+00  2.38065519   2.25956884
Mi.1.24h  3.2320262  4.4028268 -3.728132e-01  5.36656061  -1.15400984
Tr.1.24h  5.6656184  0.1697151 -1.153249e+00  2.26453500  -1.41838160
Ve.1.24h -5.2980203 -6.7050783  1.236662e+00 -4.54189220   3.22413002
Da.2.3h  -0.8314310  1.5867241  1.726763e+00 -2.54018207  -0.65644224
Do.2.3h   2.3851032 -3.1029767  6.304268e-02  1.31503254  -7.90174536
Ep.2.3h   5.2482158  2.7662966  2.313072e-01  1.88295018   1.98448903
Mi.2.3h  -6.9604250 -5.3601185  1.215431e+00 -0.14671092   3.81897543
Tr.2.3h  -0.7654477  2.1647363 -8.151810e-01  0.89588463   0.36536355
Ve.2.3h   1.0795254  2.7380019 -9.241734e-01 -4.06516015   4.63673754
Da.2.24h -0.1689449 -1.4115333 -3.710883e-01 -0.29601109   0.67098294
Do.2.24h -0.1993941  0.7517837  6.483318e-05  0.33050136  -0.32301228
Ep.2.24h -0.0374983  0.1305521  2.102847e-03 -0.22718480   0.05369490
Mi.2.24h  6.1487510  1.8818534  1.991177e+00 -0.37771777   0.28834194
Tr.2.24h -7.3368254 -0.9850691 -6.133359e+00  0.52887864  -4.60728260
Ve.2.24h  0.9451316 -1.0232908  2.025192e+00  2.57683348   1.54824528
Da.3.3h  -1.2176443  0.6747078  6.961751e-01 -0.20735564  -0.94286987
Do.3.3h   0.7110620  5.5313596 -3.560090e-01  3.51828519   5.78558611
Ep.3.3h  -5.4986450 -6.7836875 -4.931664e+00  0.14262718  -2.43762737
Mi.3.3h   4.8445532  6.4412332 -1.716751e+00 -3.29327371   0.11662076
Tr.3.3h  -2.7042134 -3.7084858  3.621754e+00 -3.81003265  -8.66836474
Ve.3.3h   1.7424755 -0.8682916  3.069675e+00  4.21755084   3.19376987
Da.3.24h -0.6325083  1.9696778 -1.210004e-02 -0.49543820  -0.88746675
Do.3.24h  0.3347110 -1.5493123  1.837425e-01  0.99138221   0.06427518
Ep.3.24h  1.3909571  1.3806904  4.884828e-01 -1.24008874  -0.63759657
Mi.3.24h -0.3399976 -2.7838249 -1.863984e+00  2.15136427  -0.30183922
Tr.3.24h  1.1421660  0.2632412 -7.113833e-01 -1.84125191   3.47334148
Ve.3.24h  0.5026603 -0.6965614  5.866082e-01 -0.18824346   0.33974541
Da.4.3h  -2.0516823 -1.3466158 -2.423308e+00 -1.37297490  -3.74383374
Do.4.3h  -1.9954948  0.8298698  1.733865e+00 -1.33283167  -1.38892274
Ep.4.3h   1.9283441  2.1197218 -2.172219e+00 -2.05207138   1.26271050
Mi.4.3h  -0.5036366  3.8911189  9.076706e+00 -0.16125131   0.23616430
Tr.4.3h   0.7115816 -2.6036717  3.436080e-01  0.60685899  -1.11757294
Ve.4.3h   2.9819965 -4.6475073 -6.869613e+00  4.05123283   7.75350691
Da.4.24h -1.3784749  3.0093917  1.782194e+00  2.36152469  -3.62803847
Do.4.24h -3.5407235 -3.7395183  1.724872e-01  0.74602507   5.73258525
Ep.4.24h  3.0887527  2.4294269 -1.801432e+00 -0.92563311  -1.65478690
Mi.4.24h  1.0216165 -8.0171166  2.411034e+00 -8.54633773   2.62379595
Tr.4.24h  4.4535333  1.3366607 -2.185493e+00  2.58736667  -0.44816959
Ve.4.24h -4.8408913  7.2306947  1.194999e+00  4.07924076  -4.51054796
Da.5.3h   2.1605354  2.4854400  7.555385e-01 -0.32139031   0.08840873
Do.5.3h   2.8486525 -4.8650917 -1.880245e+00  1.02112613  -0.43236195
Ep.5.3h  -6.1667736  8.3258588  4.239787e+00 -6.59560075   1.03419051
Mi.5.3h   1.9662671 -9.8113846 -4.077851e+00  8.39099949  -2.32188266
Tr.5.3h  -2.2072003  0.1153823 -1.666235e+00  0.44346649   2.77083663
Ve.5.3h   0.8816587  1.5423983  6.174138e-01  0.06169704  -0.64241682
Da.5.24h  0.5167687  0.3724242 -2.686338e-01 -0.50884708  -0.55979136
Do.5.24h -0.6275354 -0.5655578 -6.145990e-01  0.72650300   0.83944625
Ep.5.24h  2.3370641  0.5761364  5.487870e-01  0.46019091  -1.38891185
Mi.5.24h -3.0514184  0.6254223 -6.285316e-01 -0.46955971   0.03014470
Tr.5.24h  7.1548460  1.7618419  7.552050e+00  0.29507932   3.87003768
Ve.5.24h -6.6489504  0.1824649 -4.582801e+00 -3.41426321  -3.31156452
Da.6.3h   7.4051053 -4.0743639 -1.793605e-01  2.17339484  -1.78364595
Do.6.3h   2.7132632 -8.2048884  6.549315e+00 -7.28234789  -3.18356682
Ep.6.3h  -5.7238718  3.2066818  3.469951e+00 13.96515356   1.46060380
Mi.6.3h  -4.7872015  4.2320847  2.783108e-01 -6.43417695   8.49620242
Tr.6.3h   3.0417877  1.2380040 -2.571191e+00 -3.12463496  -5.69444601
Ve.6.3h  -0.5534063  1.6929255 -9.849188e+00  2.29789828  -1.63837445
Da.6.24h -0.6987422 -3.6393203 -3.974154e-01 -1.53763604   0.79078962
Do.6.24h -1.5531481  1.7315653  7.968576e-01  0.21023107   0.65512547
Ep.6.24h  1.4190623 -0.5363061 -1.023606e+00  0.62734971  -0.20543487
Mi.6.24h -7.5956581  4.2440881 -1.643676e+00  0.49595384  -0.41018593
Tr.6.24h -0.4363278  0.8326196 -2.175188e+00  1.63413828   1.22210828
Ve.6.24h  6.9633232 -2.9705625  6.759831e+00 -3.47046039   0.79709690
                PC62        PC63         PC64         PC65         PC66
Da.1.3h   0.15681915  1.66983587   0.65864616  -0.56746540 -0.339554928
Do.1.3h   2.37248263 -6.05961102  11.55212342  -0.41951906  1.611223257
Ep.1.3h  -2.84899266 -6.49515904  -5.58936815   2.26484587  2.651536029
Mi.1.3h  -6.21200799 10.57469614  -1.87116148  -4.38750931 -6.066132296
Tr.1.3h  -0.42873964  1.55505041  -3.35165650   2.20082737 -0.617777295
Ve.1.3h   5.93972927 -0.32381369   0.80388577   2.40603707  0.379756456
Da.1.24h -2.35768468  1.83226316   0.83607421   4.29223683 -0.092545345
Do.1.24h  2.08675080 -0.72067195  -0.47130204  -3.12794042  0.217251819
Ep.1.24h  1.00477753  0.01406533  -0.98977694  -1.55839930  0.061645984
Mi.1.24h -2.20832239 -5.94793068   0.01359406  -0.15075878  0.020426622
Tr.1.24h  6.03433390  5.07886293   8.74186052   0.55275360  1.936292552
Ve.1.24h -3.27174439 -2.24064848 -10.79823044  -1.43444208  0.139340714
Da.2.3h  -0.33277690 -0.74664563   1.12498251  -0.65982829  0.969354833
Do.2.3h  -5.16757776  0.14018870  -2.70761355   0.13976136  1.304706011
Ep.2.3h  -2.93303094  3.34782881   0.32901901   0.38634853  0.421413944
Mi.2.3h   7.14367167 -4.65394145   2.36607951  -1.51481429 -1.346358117
Tr.2.3h   0.94156671  0.26457833  -0.25930718   1.08537080 -0.847500557
Ve.2.3h   0.20738010  1.59668062   0.65826936   0.08363599 -0.708071332
Da.2.24h -1.10702679  0.93823052  -0.54591730   0.70249200  0.405660895
Do.2.24h  0.23906212 -0.44193211   0.38197616  -0.09305300  0.097720948
Ep.2.24h  0.07003761  0.13656541   0.01355287   0.04817386  0.127313514
Mi.2.24h  0.88186666 -1.89835810   0.96183768  -0.01513606 -1.276749863
Tr.2.24h -1.24200457  0.22503667  -3.44313174   1.09684214 -0.309113808
Ve.2.24h  1.17297602  1.20274810   1.77035979  -0.71435944  1.239113371
Da.3.3h  -0.57708510 -1.13077387   1.88266712   1.04357130  0.913545797
Do.3.3h   5.16373398  5.36937465  -5.00076706   1.85579406 -3.846999741
Ep.3.3h   3.93114693 -0.98603355   0.83311346   0.29622684 -3.104202956
Mi.3.3h  -2.16580997  1.23826579  -2.23195203  -0.35310951  1.064897195
Tr.3.3h  -3.22223878 -6.87348454   4.52367920  -0.69737764  6.580380982
Ve.3.3h  -2.14494650  2.24810349  -1.18862558  -2.64676412  0.962641063
Da.3.24h -2.57328696  0.66354777   1.94208678   2.97202454  0.572782903
Do.3.24h  0.49177808 -0.03521155   0.21601865  -1.04707074  0.174854482
Ep.3.24h  1.99608486 -0.67952921  -0.86095509  -1.77416766 -0.399852885
Mi.3.24h  0.30622192 -1.79338305  -0.25464662  -0.30112587 -0.773488682
Tr.3.24h -1.25436109  1.59373024  -0.16058884  -1.08577472 -2.512280522
Ve.3.24h -0.03327675  0.36291992  -0.25171553   1.01128958 -0.344096887
Da.4.3h  -1.12239698  5.91344258   7.49906847  -2.18124512 -3.725586937
Do.4.3h   3.33669932 -1.31360435  -1.22560440   2.54068092  5.269705147
Ep.4.3h   1.85180560 -3.41147412  -7.39841487   1.01226220  1.548538546
Mi.4.3h   0.87684549 -1.86114103  -2.59050622   4.00484675  6.272775897
Tr.4.3h   1.56495041 -1.94883486   0.89088430   2.31897697 -3.035591505
Ve.4.3h  -8.82876321 -0.76155076   0.30690119  -9.06186218 -7.317710837
Da.4.24h  8.32184858 -5.70885422  -2.01220908 -13.48989562 -1.329663126
Do.4.24h -7.03105953  3.36284109   1.14441667  11.74829763  0.014047497
Ep.4.24h -3.07652319  0.51327154   2.02902789   1.82149483  0.287279238
Mi.4.24h  7.11389041 10.29741256  -1.32736928  -1.56062465  0.556342778
Tr.4.24h  0.00700678  1.17727864   2.37531867   0.24378173  9.004581284
Ve.4.24h -2.28384956 -5.93932973   0.52495439   2.04767461 -6.440513402
Da.5.3h  -3.54165154  0.69572191   1.38035274  -0.82879639  2.050376050
Do.5.3h   0.44789721 -1.77385732  -0.67263114  -0.95078930 -1.482184435
Ep.5.3h   2.22590945  2.43012300  -0.17890022  -1.11280095 -3.030325643
Mi.5.3h   1.28812175 -1.96651722   0.60508347   3.14991190  0.329660950
Tr.5.3h  -1.74946807  0.33539271  -0.68329114  -1.49353661  1.239359672
Ve.5.3h  -0.40733830 -0.14621785  -0.22224320   0.87484080 -0.225627452
Da.5.24h -0.16649519  0.27739562   0.03685670  -0.46133809 -0.012728846
Do.5.24h  1.00941609  0.67438692   0.35130209  -0.01381281 -0.522616765
Ep.5.24h  0.21879836 -2.25573163  -0.59106773  -0.63919985  0.298298357
Mi.5.24h -0.94552400  2.86438417   0.53156947   1.55772494  0.504816465
Tr.5.24h  0.41256677 -1.54098444  -0.20873786  -0.56217913 -0.650766342
Ve.5.24h  0.59887878  0.57819945  -0.45808874   0.72568158  1.136962398
Da.6.3h   3.01172748 -1.91774866  -7.97132403   2.18910953 -2.631884750
Do.6.3h  -6.35632289 -1.98207743   3.66548033  -3.14072433  3.093301417
Ep.6.3h  -1.75463717  3.07400892   4.68462755  -1.45084936  1.292772666
Mi.6.3h  -1.94831828 -1.20697866   2.20914297  -2.50139106 -0.704998005
Tr.6.3h   7.55319276 -2.52317637   1.99654466   9.58911344 -9.991278755
Ve.6.3h   4.74661449  7.24442745  -3.73227492  -3.10567387 11.055046902
Da.6.24h  0.59164219  0.85343724  -1.92031154   1.65387819  0.480740138
Do.6.24h -0.17509747 -0.31531768  -0.39627823  -2.10398601  0.273354178
Ep.6.24h  0.73760516  0.99874363   1.04290643   0.73786444 -0.824816261
Mi.6.24h -5.03143640 -2.44105297   0.66722004   0.50655566  1.097781307
Tr.6.24h  0.09722504 -0.08740788  -2.08372755  -0.05048241  0.005068316
Ve.6.24h -1.65326641 -1.21405522   2.09821191  -1.90312443 -3.151650301
                  PC67          PC68         PC69          PC70         PC71
Da.1.3h   -0.609100729  -3.104194155  0.063107204   1.897333184 -0.332510045
Do.1.3h  -10.093667994  -5.309186037 -0.971235343   3.437902608 -0.174125817
Ep.1.3h    7.408261452  10.661236546 -1.829366078  -4.447055408  1.270942265
Mi.1.3h   -0.765044846  -5.879411054  2.124253285   0.571909021  0.219221375
Tr.1.3h   -1.857053389  -0.164861925  3.606579372   0.530580948 -0.962972853
Ve.1.3h    5.021836860   3.904666001 -1.810208607  -0.165283722 -0.842720808
Da.1.24h  -0.319381732  -0.701694183 -0.673357342  -0.761331164  0.243388303
Do.1.24h   0.241287078   0.440202244  0.203063305  -0.201021803 -0.187237961
Ep.1.24h   0.117111664   0.522851041  0.490172054   1.100211046 -0.078665933
Mi.1.24h  -0.282110963   0.391894513  2.156169601   0.479840692  0.639965929
Tr.1.24h   3.373779920  -1.902422700 -5.840027954  -5.751599785  0.268587363
Ve.1.24h  -1.787410112   1.544293151  2.804653093   2.801511942  0.304013294
Da.2.3h   -0.188952198   0.767185421  0.197132998  -0.862485053  0.053256424
Do.2.3h   -0.021392815   2.355171967 -2.975388790   1.243323683  0.147549696
Ep.2.3h   -1.417365377  -1.359712802  0.617807956   0.587897249 -0.277106223
Mi.2.3h    1.638633989  -1.256272632  1.852246137  -0.623057019  0.401327864
Tr.2.3h    0.750659290  -0.077474971  0.019481047   0.230460586 -0.892141276
Ve.2.3h   -0.587121228  -0.003174709  2.035573307  -0.116842354  0.337360971
Da.2.24h   0.089093694  -0.166356681 -0.186579175   0.152886845 -0.129601276
Do.2.24h   0.179905330  -0.021181674  0.041724961  -0.033203606  0.042808557
Ep.2.24h   0.127194020  -0.018236786 -0.033352130   0.003159153 -0.026823285
Mi.2.24h  -0.219456349  -0.244652648 -0.329947314  -1.187088361  0.297471901
Tr.2.24h   0.153030451  -0.126809872 -1.668887179  -0.863850576 -0.415493973
Ve.2.24h  -0.043265564  -0.016911557  0.463271085   0.853352091  0.484453043
Da.3.3h    3.078617469  -0.658305051  0.490155472   0.209992996 -0.195663380
Do.3.3h    0.401514819   3.208518645  1.860533060   0.064546930  0.985679202
Ep.3.3h   -4.338630328   1.983517793 -2.165335335  -1.042619909  0.143321582
Mi.3.3h    0.814579780  -0.238530062  0.395800372  -0.145448438  0.841660904
Tr.3.3h    0.689930808  -3.965923355 -2.648111769  -0.894489355 -1.885821960
Ve.3.3h    0.216312271  -1.044849561  0.259939506   0.121180969 -0.705510163
Da.3.24h  -0.552665746  -0.256990748 -0.105242965  -0.378337203  0.048874182
Do.3.24h   0.003556331   0.431149365  0.180809664   1.102864006 -0.001529806
Ep.3.24h   0.445804098   0.053489068 -0.156479322  -0.589150269 -0.020525282
Mi.3.24h  -0.365064827  -0.345043302  1.738602104   0.652384048  0.341319563
Tr.3.24h  -0.117636312   1.519220825  0.201429422   0.371893030  0.922203097
Ve.3.24h   0.019474040  -0.599382212 -0.702249075   0.500407863 -0.570136363
Da.4.3h   -0.313815446  10.167295489  1.609425694   1.589172398  0.667424545
Do.4.3h    2.208580340  -4.444870751 11.123355653 -13.916976946 -4.610119753
Ep.4.3h    9.602311778 -11.416027185 -8.727990321   7.508143748 -0.955582048
Mi.4.3h  -11.104612158   7.728826899 -6.438956915   3.568379726 -3.117592282
Tr.4.3h    0.523343641  -1.244063613  2.774597365   1.009849481 17.388951287
Ve.4.3h   -0.633549611   0.364925124 -1.244753051  -1.957634103 -6.633387685
Da.4.24h   0.682633390   1.705517651  0.546921861   1.293856536 -0.163544561
Do.4.24h   0.315073814  -2.121593595 -1.472085876  -1.007321816  0.096806605
Ep.4.24h  -1.616221013  -0.190633158  0.905411781  -0.829277445  0.164307136
Mi.4.24h   0.969095691  -0.330482264 -4.312549655  -0.849982421 -1.746404357
Tr.4.24h   1.504121269   0.174855059 11.567705888  10.526979372 -1.980995273
Ve.4.24h  -2.969416202  -1.333825355 -5.795643581  -5.925571846  0.495061139
Da.5.3h    0.786682206  -0.703955244 -1.051468331   0.243077483  0.715534684
Do.5.3h   -0.411211178  -0.607881606  0.679629268   1.111295290  0.122384167
Ep.5.3h   -0.656132212   0.221878179  0.088980952   0.955365858  0.042002955
Mi.5.3h    1.976611721   1.027394624  0.098139192  -0.399717750 -0.783387600
Tr.5.3h   -0.545614116  -0.491488965  0.146905910  -0.950636551  0.343136733
Ve.5.3h   -0.076744300   0.071360688 -0.606768914  -0.156329498  0.053037801
Da.5.24h  -0.037499341   0.704187659 -0.005802818   0.173160690  0.026272467
Do.5.24h  -0.234989123  -0.495433685  0.021368717  -0.132722430 -0.069600783
Ep.5.24h  -0.078032982  -0.122891160  0.516444596  -0.034676949  0.166275288
Mi.5.24h   0.302123450  -0.129609988 -0.479290606   0.112855184 -0.294976214
Tr.5.24h  -2.078133093  -0.124029420 -1.019170001  -2.091790018  0.430097917
Ve.5.24h   1.097003868   0.848614707  1.000333906   0.840585178 -0.336650593
Da.6.3h  -11.083495736  -4.827534417  2.853381501  -2.295126737  0.482301423
Do.6.3h    3.483057671   3.515917526 -0.551233088  -0.217747981  1.099145871
Ep.6.3h    2.431498288  -1.376415725 -0.819416547   2.015417937 -0.338382729
Mi.6.3h    4.436785669  -0.690837765  0.783826536  -1.873757926  1.510313414
Tr.6.3h    3.467012512   2.453850808  2.124526689   5.673782772 -6.355405174
Ve.6.3h   -3.564476708   1.080215399 -4.348694709  -2.708929966  2.334389293
Da.6.24h   0.389550553   0.153900367 -0.182601990  -0.355521386 -0.022744615
Do.6.24h   0.056376925   0.554261975 -0.109833779   0.219247802  0.427123857
Ep.6.24h   0.594547275  -0.242502441  0.083953505   0.476920130 -0.337737628
Mi.6.24h  -0.097789243   0.815483909  0.939823002   1.254019929  0.276164997
Tr.6.24h  -0.711330489  -0.722744743 -0.436394560  -0.537946275  0.129831286
Ve.6.24h   0.181390033  -0.293482886  0.031186095  -1.181216336  0.481129320
                  PC72
Da.1.3h  -6.651229e-14
Do.1.3h  -4.489259e-14
Ep.1.3h  -9.254712e-14
Mi.1.3h  -1.628912e-13
Tr.1.3h  -1.385861e-13
Ve.1.3h  -1.559786e-13
Da.1.24h  1.348112e-13
Do.1.24h -3.666718e-13
Ep.1.24h -2.127344e-13
Mi.1.24h -1.530603e-13
Tr.1.24h -1.327780e-13
Ve.1.24h -6.613611e-14
Da.2.3h   2.174891e-13
Do.2.3h   1.314039e-13
Ep.2.3h  -4.111564e-14
Mi.2.3h   1.927072e-13
Tr.2.3h   8.026929e-14
Ve.2.3h   7.455985e-14
Da.2.24h -2.085120e-13
Do.2.24h -1.400767e-13
Ep.2.24h -2.269504e-13
Mi.2.24h  1.164443e-13
Tr.2.24h  1.717236e-13
Ve.2.24h  1.082040e-13
Da.3.3h   5.683559e-14
Do.3.3h  -1.338923e-14
Ep.3.3h   1.053792e-14
Mi.3.3h   1.030323e-13
Tr.3.3h   1.818786e-13
Ve.3.3h   2.986194e-14
Da.3.24h -5.555761e-14
Do.3.24h -1.135552e-13
Ep.3.24h -2.581390e-13
Mi.3.24h -4.964779e-15
Tr.3.24h  1.111992e-13
Ve.3.24h  9.766046e-14
Da.4.3h  -8.011418e-14
Do.4.3h  -8.794967e-15
Ep.4.3h  -8.694526e-14
Mi.4.3h  -9.490188e-14
Tr.4.3h   7.359714e-14
Ve.4.3h  -2.669925e-14
Da.4.24h -5.633543e-13
Do.4.24h -2.208718e-13
Ep.4.24h -1.101381e-13
Mi.4.24h -7.260077e-14
Tr.4.24h  1.473639e-14
Ve.4.24h -2.136728e-14
Da.5.3h   8.228456e-14
Do.5.3h   5.982603e-14
Ep.5.3h   1.444372e-13
Mi.5.3h   6.829189e-14
Tr.5.3h   1.645843e-13
Ve.5.3h   1.453787e-13
Da.5.24h  8.805031e-14
Do.5.24h  5.840459e-14
Ep.5.24h -2.688076e-14
Mi.5.24h  2.896994e-14
Tr.5.24h  1.365049e-13
Ve.5.24h  1.668764e-13
Da.6.3h  -6.140879e-14
Do.6.3h  -1.924612e-14
Ep.6.3h  -6.040741e-15
Mi.6.3h   5.698397e-14
Tr.6.3h   8.373978e-14
Ve.6.3h   1.407447e-13
Da.6.24h -1.038608e-13
Do.6.24h -2.735874e-14
Ep.6.24h -9.366458e-14
Mi.6.24h  1.902084e-14
Tr.6.24h  6.407533e-14
Ve.6.24h  3.958598e-14
function(pca) {
  # x: class == prcomp
  pca.var <- pca$sdev^2
  pca.prop <- pca.var / sum(pca.var)
  var.plot <- qplot(PC, prop, data = data.frame(PC = 1:length(pca.prop),
                                                prop = pca.prop)) +
    labs(title = 'Variance contributed by each PC',
         x = 'PC', y = 'Proportion of variance')
}

Variance contribution from treatment, extraction time, or individual on PC1 and PC2

Version Author Date
c2db62c reneeisnowhere 2023-06-16
cdd9374 reneeisnowhere 2023-02-10

Version Author Date
cdd9374 reneeisnowhere 2023-02-10

Version Author Date
c2db62c reneeisnowhere 2023-06-16
cdd9374 reneeisnowhere 2023-02-10

Version Author Date
cdd9374 reneeisnowhere 2023-02-10

Version Author Date
c2db62c reneeisnowhere 2023-06-16
cdd9374 reneeisnowhere 2023-02-10

Version Author Date
cdd9374 reneeisnowhere 2023-02-10

Warning: Using shapes for an ordinal variable is not advised

Version Author Date
c2db62c reneeisnowhere 2023-06-16

Differential Expression

Version Author Date
cdd9374 reneeisnowhere 2023-02-10
                 Length Class      Mode     
coefficients     140840 -none-     numeric  
stdev.unscaled   140840 -none-     numeric  
sigma             14084 -none-     numeric  
df.residual       14084 -none-     numeric  
cov.coefficients    100 -none-     numeric  
pivot                12 -none-     numeric  
rank                  1 -none-     numeric  
genes                 2 data.frame list     
Amean             14084 -none-     numeric  
method                1 -none-     character
design              864 -none-     numeric  
contrasts           120 -none-     numeric  
df.prior              1 -none-     numeric  
s2.prior              1 -none-     numeric  
var.prior            10 -none-     numeric  
proportion            1 -none-     numeric  
s2.post           14084 -none-     numeric  
t                140840 -none-     numeric  
df.total          14084 -none-     numeric  
p.value          140840 -none-     numeric  
lods             140840 -none-     numeric  
F                 14084 -none-     numeric  
F.p.value         14084 -none-     numeric  
mm2 <- model.matrix(~0 + group1)
##made the matrix model using the interaction between Treatment and Time
colnames(mm2) <- c("A3", "X3", "E3","M3","T3", "V3","A24", "X24", "E24","M24","T24", "V24")
y2 <- voom(x, mm2)

corfit2 <- duplicateCorrelation(y2, mm2, block = indv)

v2 <- voom(x, mm2, block = indv, correlation = corfit2$consensus)

fit2 <- lmFit(v2, mm2, block = indv, correlation = corfit2$consensus)

vfit2 <- lmFit(v2, mm2)

vfit2<- contrasts.fit(vfit2, contrasts=cm2)

efit2 <- eBayes(vfit2)

V.DA.top= topTable(efit2, coef=1, adjust="BH", number=Inf, sort.by="p")
### sorting all top expressed genes for the Vehicle and Daunorubicin 3 hour treatments
sigVDA3 = V.DA.top[V.DA.top$adj.P.Val < .1 , ]
### this helped pull only those files that were at an adjusted p value of less than 0.1
### This p-value was used as the beginning examination of the data, considering I will run multiple runs of this RNA seq library.

This is the example code I used to process my data. I used two model matrix initially, one set up was /~0 +drug +time and the second was /~0+group1, then blocking by individual. That is why you see the number 2 in the code above.

#DEG summary

Importance of components:
                           PC1     PC2      PC3      PC4      PC5      PC6
Standard deviation     62.4366 46.4535 34.03056 32.38995 28.09502 24.20110
Proportion of Variance  0.2768  0.1532  0.08223  0.07449  0.05604  0.04159
Cumulative Proportion   0.2768  0.4300  0.51224  0.58673  0.64277  0.68436
                            PC7      PC8      PC9     PC10     PC11    PC12
Standard deviation     22.20405 20.69847 19.78302 18.00510 17.09551 16.2307
Proportion of Variance  0.03501  0.03042  0.02779  0.02302  0.02075  0.0187
Cumulative Proportion   0.71936  0.74978  0.77757  0.80059  0.82134  0.8400
                           PC13     PC14     PC15     PC16     PC17     PC18
Standard deviation     14.42210 13.35826 12.24616 11.57513 10.76393 10.45848
Proportion of Variance  0.01477  0.01267  0.01065  0.00951  0.00823  0.00777
Cumulative Proportion   0.85481  0.86748  0.87813  0.88764  0.89587  0.90363
                          PC19    PC20    PC21    PC22    PC23    PC24    PC25
Standard deviation     9.40687 8.97456 8.75212 8.20930 7.94130 7.34670 6.96962
Proportion of Variance 0.00628 0.00572 0.00544 0.00479 0.00448 0.00383 0.00345
Cumulative Proportion  0.90992 0.91564 0.92107 0.92586 0.93034 0.93417 0.93762
                          PC26    PC27    PC28    PC29    PC30    PC31    PC32
Standard deviation     6.65895 6.54610 6.13447 6.10142 6.01330 5.71516 5.57713
Proportion of Variance 0.00315 0.00304 0.00267 0.00264 0.00257 0.00232 0.00221
Cumulative Proportion  0.94077 0.94381 0.94648 0.94913 0.95169 0.95401 0.95622
                          PC33    PC34    PC35    PC36   PC37    PC38    PC39
Standard deviation     5.50168 5.35324 5.21994 5.15023 4.8971 4.86687 4.71568
Proportion of Variance 0.00215 0.00203 0.00193 0.00188 0.0017 0.00168 0.00158
Cumulative Proportion  0.95837 0.96040 0.96234 0.96422 0.9659 0.96761 0.96919
                          PC40    PC41    PC42    PC43    PC44    PC45    PC46
Standard deviation     4.65993 4.53939 4.50666 4.29982 4.25872 4.15079 4.07068
Proportion of Variance 0.00154 0.00146 0.00144 0.00131 0.00129 0.00122 0.00118
Cumulative Proportion  0.97073 0.97219 0.97363 0.97495 0.97623 0.97746 0.97863
                          PC47    PC48    PC49    PC50    PC51    PC52   PC53
Standard deviation     4.03193 3.98075 3.95315 3.90338 3.85976 3.81543 3.7552
Proportion of Variance 0.00115 0.00113 0.00111 0.00108 0.00106 0.00103 0.0010
Cumulative Proportion  0.97979 0.98091 0.98202 0.98310 0.98416 0.98520 0.9862
                          PC54    PC55    PC56    PC57   PC58    PC59    PC60
Standard deviation     3.68512 3.65754 3.62646 3.58982 3.5642 3.49341 3.46598
Proportion of Variance 0.00096 0.00095 0.00093 0.00091 0.0009 0.00087 0.00085
Cumulative Proportion  0.98716 0.98811 0.98904 0.98996 0.9909 0.99173 0.99258
                          PC61    PC62    PC63    PC64   PC65    PC66    PC67
Standard deviation     3.40572 3.33298 3.27132 3.23877 3.1490 3.09861 3.07446
Proportion of Variance 0.00082 0.00079 0.00076 0.00074 0.0007 0.00068 0.00067
Cumulative Proportion  0.99340 0.99419 0.99495 0.99570 0.9964 0.99708 0.99775
                          PC68    PC69    PC70    PC71     PC72
Standard deviation     2.97300 2.88995 2.84338 2.51996 4.02e-14
Proportion of Variance 0.00063 0.00059 0.00057 0.00045 0.00e+00
Cumulative Proportion  0.99838 0.99898 0.99955 1.00000 1.00e+00
        V.DA  V.DX  V.EP  V.MT  V.TR V.DA24 V.DX24 V.EP24 V.MT24 V.TR24
Down     109     3    30    24     0   3540   3336   3105    428      0
NotSig 13552 14065 13874 14009 14084   7067   7439   7756  12969  14084
Up       423    16   180    51     0   3477   3309   3223    687      0

Graphing DE genes

I then created a counts table for each set of genes. Luckily, the counts are stored in the y2 object, which is an EList class object. I can ‘simplify’ this process because I kept the DEGList format initially. I first made an object called ‘countstotal’ from the EList y2. For ggploting later, I subsetted ‘countstotal’ by treatments.

Version Author Date
cdd9374 reneeisnowhere 2023-02-10

library(cowplot)

Volcano Plots

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c2db62c reneeisnowhere 2023-06-16
64c9be0 reneeisnowhere 2023-02-10
cdd9374 reneeisnowhere 2023-02-10

Version Author Date
c2db62c reneeisnowhere 2023-06-16
64c9be0 reneeisnowhere 2023-02-10
cdd9374 reneeisnowhere 2023-02-10

Version Author Date
c2db62c reneeisnowhere 2023-06-16
64c9be0 reneeisnowhere 2023-02-10
cdd9374 reneeisnowhere 2023-02-10

Version Author Date
c2db62c reneeisnowhere 2023-06-16
64c9be0 reneeisnowhere 2023-02-10
cdd9374 reneeisnowhere 2023-02-10

Version Author Date
c2db62c reneeisnowhere 2023-06-16
64c9be0 reneeisnowhere 2023-02-10
cdd9374 reneeisnowhere 2023-02-10

Version Author Date
cdd9374 reneeisnowhere 2023-02-10

Version Author Date
cdd9374 reneeisnowhere 2023-02-10

Version Author Date
cdd9374 reneeisnowhere 2023-02-10

GO analysis


R version 4.3.1 (2023-06-16 ucrt)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows 10 x64 (build 19045)

Matrix products: default


locale:
[1] LC_COLLATE=English_United States.utf8 
[2] LC_CTYPE=English_United States.utf8   
[3] LC_MONETARY=English_United States.utf8
[4] LC_NUMERIC=C                          
[5] LC_TIME=English_United States.utf8    

time zone: America/Chicago
tzcode source: internal

attached base packages:
[1] stats4    stats     graphics  grDevices utils     datasets  methods  
[8] base     

other attached packages:
 [1] pheatmap_1.0.12                        
 [2] ggfortify_0.4.16                       
 [3] Hmisc_5.1-1                            
 [4] corrplot_0.92                          
 [5] ggrepel_0.9.4                          
 [6] cowplot_1.1.1                          
 [7] Homo.sapiens_1.3.1                     
 [8] TxDb.Hsapiens.UCSC.hg19.knownGene_3.2.2
 [9] org.Hs.eg.db_3.17.0                    
[10] GO.db_3.17.0                           
[11] OrganismDbi_1.42.0                     
[12] GenomicFeatures_1.52.2                 
[13] GenomicRanges_1.52.1                   
[14] GenomeInfoDb_1.36.4                    
[15] AnnotationDbi_1.62.2                   
[16] IRanges_2.34.1                         
[17] S4Vectors_0.38.2                       
[18] Biobase_2.60.0                         
[19] biomaRt_2.56.1                         
[20] scales_1.3.0                           
[21] lubridate_1.9.3                        
[22] forcats_1.0.0                          
[23] stringr_1.5.0                          
[24] dplyr_1.1.3                            
[25] purrr_1.0.2                            
[26] readr_2.1.4                            
[27] tidyr_1.3.0                            
[28] tibble_3.2.1                           
[29] ggplot2_3.4.4                          
[30] tidyverse_2.0.0                        
[31] AnnotationHub_3.8.0                    
[32] BiocFileCache_2.8.0                    
[33] dbplyr_2.4.0                           
[34] BiocGenerics_0.46.0                    
[35] devtools_2.4.5                         
[36] usethis_2.2.2                          
[37] reshape2_1.4.4                         
[38] gridExtra_2.3                          
[39] RColorBrewer_1.1-3                     
[40] edgeR_3.42.4                           
[41] limma_3.56.2                           
[42] workflowr_1.7.1                        

loaded via a namespace (and not attached):
  [1] later_1.3.1                   BiocIO_1.10.0                
  [3] bitops_1.0-7                  filelock_1.0.3               
  [5] graph_1.78.0                  rpart_4.1.23                 
  [7] XML_3.99-0.16.1               lifecycle_1.0.4              
  [9] rprojroot_2.0.4               vroom_1.6.5                  
 [11] processx_3.8.2                lattice_0.22-5               
 [13] backports_1.4.1               magrittr_2.0.3               
 [15] sass_0.4.7                    rmarkdown_2.25               
 [17] jquerylib_0.1.4               yaml_2.3.7                   
 [19] remotes_2.4.2.1               httpuv_1.6.12                
 [21] sessioninfo_1.2.2             pkgbuild_1.4.3               
 [23] DBI_1.2.1                     abind_1.4-5                  
 [25] pkgload_1.3.4                 zlibbioc_1.46.0              
 [27] RCurl_1.98-1.14               nnet_7.3-19                  
 [29] rappdirs_0.3.3                git2r_0.32.0                 
 [31] GenomeInfoDbData_1.2.10       codetools_0.2-19             
 [33] DelayedArray_0.26.7           xml2_1.3.5                   
 [35] tidyselect_1.2.0              farver_2.1.1                 
 [37] base64enc_0.1-3               matrixStats_1.1.0            
 [39] GenomicAlignments_1.36.0      jsonlite_1.8.7               
 [41] ellipsis_0.3.2                Formula_1.2-5                
 [43] tools_4.3.1                   progress_1.2.3               
 [45] Rcpp_1.0.11                   glue_1.6.2                   
 [47] xfun_0.41                     MatrixGenerics_1.12.3        
 [49] withr_3.0.0                   BiocManager_1.30.22          
 [51] fastmap_1.1.1                 fansi_1.0.5                  
 [53] callr_3.7.3                   digest_0.6.33                
 [55] timechange_0.2.0              R6_2.5.1                     
 [57] mime_0.12                     colorspace_2.1-0             
 [59] RSQLite_2.3.5                 utf8_1.2.4                   
 [61] generics_0.1.3                data.table_1.14.8            
 [63] rtracklayer_1.60.1            prettyunits_1.2.0            
 [65] httr_1.4.7                    htmlwidgets_1.6.4            
 [67] S4Arrays_1.0.6                whisker_0.4.1                
 [69] pkgconfig_2.0.3               gtable_0.3.4                 
 [71] blob_1.2.4                    XVector_0.40.0               
 [73] htmltools_0.5.7               profvis_0.3.8                
 [75] RBGL_1.76.0                   png_0.1-8                    
 [77] knitr_1.45                    rstudioapi_0.15.0            
 [79] tzdb_0.4.0                    rjson_0.2.21                 
 [81] checkmate_2.3.1               curl_5.2.0                   
 [83] cachem_1.0.8                  BiocVersion_3.17.1           
 [85] parallel_4.3.1                miniUI_0.1.1.1               
 [87] foreign_0.8-86                restfulr_0.0.15              
 [89] pillar_1.9.0                  grid_4.3.1                   
 [91] vctrs_0.6.4                   urlchecker_1.0.1             
 [93] promises_1.2.1                cluster_2.1.4                
 [95] xtable_1.8-4                  htmlTable_2.4.2              
 [97] evaluate_0.23                 cli_3.6.1                    
 [99] locfit_1.5-9.8                compiler_4.3.1               
[101] Rsamtools_2.16.0              rlang_1.1.2                  
[103] crayon_1.5.2                  labeling_0.4.3               
[105] ps_1.7.5                      getPass_0.2-2                
[107] plyr_1.8.9                    fs_1.6.3                     
[109] stringi_1.7.12                viridisLite_0.4.2            
[111] BiocParallel_1.34.2           munsell_0.5.0                
[113] Biostrings_2.68.1             Matrix_1.6-2                 
[115] hms_1.1.3                     bit64_4.0.5                  
[117] statmod_1.5.0                 KEGGREST_1.40.1              
[119] shiny_1.8.0                   highr_0.10                   
[121] SummarizedExperiment_1.30.2   interactiveDisplayBase_1.38.0
[123] memoise_2.0.1                 bslib_0.6.1                  
[125] bit_4.0.5